食管鳞癌组织中survivin、MGMT蛋白的表达及其临床病理意义

目的 探讨凋亡抑制基因survivin、DNA修复酶MGMT在食管鳞癌组织中的表达以及与临床病理因素之间的关系.方法 应用免疫组织化学法(EnVision二步法),检测51例汉族食管鳞状细胞癌组织和18例正常切缘食管粘膜中survivin和MGMT蛋白的表达并分析其与临床病理诸因素之间的关系.结果 survivin在18例正常切缘食管组织中不表达,在51例食管鳞癌组织中34例表达阳性,占66.67%.MGMT在食管鳞癌组织中的表达(80.39%)高于切缘正常食管粘膜(16.67%)(P<0.01);survivin,MGMT蛋白阳性表达率与食管鳞癌的浸润深度、TNM分期有关,差异有统计学意义(P<0.05).此外,survivin在高、中分化组与低分化组中的阳性表达率分别为56.80%和92.00%,两者差异有统计学意义(P<0.05),但MGMT蛋白表达阳性率与肿瘤分化程度无相关性.survivin与MGMT蛋白表达均与食管鳞癌的浸润深度及TNM分期有关(P均<0.05),而与患者年龄、性别、肿瘤部位、肿瘤大小、大体标本分型及有无淋巴结转移等因素无关(P>0.05);survivin和MGMT蛋白在食管鳞癌组织中的表达无相关性(P>0.05).结论 survivin、MGMT基因在食管鳞癌组织中异常表达并与食管鳞癌组织的分化程度、浸润深度、TNM分期密切相关.

Expression and clinical pathological significance of survivin and MGMT in esophageal squamous cell carcinoma

Objective To investigate the expression and clinical significance of survivin and MGMT protein in esophageal squamous cell carcinoma(ESCC). Methods The presence and the level of expression of survivin and MGMT protein in 51 cases of ESCC and 18 cases of the normal tissue along the incision margin (TIM) were detected by immunohistochemical techniques (EnVision). Results Survivin protein was not detected in TIM,but there were 34 positive case in 51 cases (66.67%). There was significant difference between the ...