经颅电刺激对脑梗死大鼠的运动功能及神经微丝表达的影响

目的　研究经颅电刺激治疗对大鼠脑梗死后运动功能、神经微丝蛋白 (NFP 2 0 0 )表达及脑可塑性物质基础的影响机制。方法　SD成年雄性大鼠大脑中动脉闭塞 (MCAO)后 ,经颅电刺激大鼠双眼脑间到人字缝处相应的双侧感觉运动区 ,以横木行走试验 (BWT)评价大鼠的精细运动功能恢复情况 ,免疫组化法观察各时段缺血性半影区神经元轴突变化及NFP 2 0 0的表达。结果　经颅电刺激治疗的大鼠 ,运动功能的恢复较对照组明显改善 (P <0 .0 1)。治疗组第 1个疗程末缺血性半影区NFP 2 0 0阳性神经轴突的形态学变化及表达与对照组相似 (P >0 .0 5 ) ,从 2～ 6个疗程缺血性半影区阳性纤维的表达均较对照组高 (P <0 .0 5 )。结论　经颅电刺激可以改善急性脑梗死大鼠瘫痪肢体运动功能 ,可能与以下机制有关 :经颅电刺激直接兴奋大脑皮质的运动中枢 ,形成神经冲动 ,引起相应大脑皮质神经元发生可塑性变化 ,促进中枢神经轴突发芽及形成新的神经联系 ,从而使兴奋从大脑皮质传导到骨骼肌乃至整个运动系统。

Effect of transcranial electric stimulation on motor function and neurofibrillar expression in rat with brain in farction

Objective To study the effect of transcranial electric stimulation (TES) on motor function and neurofibrillar (NF200) expression in rat with brain infarction and to explore mechanism of brain plasticity. Methods The infarction model was established by middle cerebral artery occlusion (MCAO) in adult Sprague Dawley male rats. Transcranial electric stimulation of sensory motor areas of both sides was applied, and the recovery of fine motor function of rat was reflected with beam walking test and the change of neurofilament protein (NFP 200) expression examined. Results The recovery of motor function of the rats treated with TES was significantly better than the controls ( P <0.01). At the end of the first week, NFP 200 expression was similar in rats of the TES and the control groups. From the 2nd to 6th week of TES treatment, the expression of NFP 200 in the ischemic penumbra was higher than that of the control group ( P <0.05). Conclusion TES can improve the motor function of the paralyzed limbs, probably due to direct effects of TES on the plasticity of the motor center of cerebral cortex.