槲皮素和乙酸龙脑酯对细菌脂多糖诱导流产孕小鼠子宫组织中CD4+/CD8+ T细胞的影响

目的 探讨小鼠子宫组织中CD4⁺ 和CD8⁺ T细胞在早期胚胎丢失中的意义，研究槲皮素和乙酸龙脑酯对细菌脂多糖（LPS）诱导流产小鼠的保胎效果和对母胎界面免疫平衡的调节作用。 方法 采用LPS尾静脉注射，制造小鼠流产模型，孕4～7d分别口服不同保胎药物，用药前后检测各组（n=10）小鼠子宫组织CD4⁺/CD8⁺T细胞亚群的变化。 结果 LPS促流产后，小鼠子宫壁CD4+T细胞数量显著增多（P<0.01），CD8⁺ T细胞无明显变化（P>0.05），CD4⁺/CD8⁺比值显著升高（P<0.01）。预先口服保胎药物能不同程度抑制LPS的作用。其中以槲皮素和乙酸龙脑酯联合使用组的保胎效果最为明显，CD4^+/CD8⁺比值下降，较LPS流产模型组差异极显著（P<0.01）。 结论 小鼠子宫组织CD4^+/CD8⁺比值升高与早期胚胎丢失关系密切，槲皮素和乙酸龙脑酯能够调节小鼠子宫局部的免疫微环境，从而起到一定的促孕保胎作用。

INFLUENCE OF QUERCETIN AND BORNYL ACETATE ON CD4SUP>+ /SUP>/CD8SUP>+/SUP>T LYMPHOCYTES IN THE UTERUS FOLLOWING LPS INDUCED ABORTION IN PREGNANT MICE

Objective To investigate the significance of CD4SUP>+ /SUP>and CD8SUP>+/SUP> T lymphocytes in the uterus in early embryo loss (or resorption), and to elucidate the anti-bortive effect and the immunological modulation of the maternal fetal interaction with quercetin and bornyl acetate. Methods Lipopolysaccharide (LPS)（0.10μg/mouse）was injected via the tail vein in order to induce abortion in 7-day-gestation mice which also received quercetin and bornyl acetate at days 4-7 of gestation. Uterine CD4SUP>+/SUP> and CD8SUP>+/SUP> T lymphocytes of each group (EM>n/EM>=10) were detected by immunohistochemistry. Results The amount of CD4EM>SUP>+ /SUP>/EM>T lymphocytes in the uterus of LPS induced abortion mice was much higher (EM>P/EM><0.01) than that of the control mice, but there was no significant difference in the amount of CD4SUP>+ /SUP>T lymphocytes (EM>P/EM>>0.05). Also, the rate of CD4SUP>+/SUP> /CD8SUP>+/SUP> was increased significantly (P<0.01). When quercetin and bornyl acetate was used to prevent LPS induced abortion, fewer CD4SUP>+/SUP> T cells were counted. The effect of quercetin combined with bornyl on anti LPS induced abortion was more significant, and the rate of CD4SUP>+ /SUP>/