An improved method for recovery of F-defective Sendai virus expressing foreign genes from cloned cDNA |
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Authors: | Hirata Takahiro Iida Akihiro Shiraki-Iida Takako Kitazato Kaio Kato Atsushi Nagai Yoshiyuki Hasegawa Mamoru |
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Institution: | DNAVEC Research Inc., 1-25-11 Kannondai, Tsukuba-shi, Ibaraki 305-0856, Japan. |
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Abstract: | An improved system is described to recover non-transmissible Sendai virus that lack the envelope fusion (F) gene from cloned cDNA. The system (1) used plasmids that expressed the F and the HN viral envelope proteins, as well as the plasmids that expressed the viral NP, P, and L proteins as helper plasmids for recovery, and (2) overlaid packaging cells that expressed the F protein. With this improved system, we have succeeded in recovery of F-defective Sendai virus expressing two foreign proteins, and expression vectors that do not contain the EGFP reporter gene. This system may provide the basis for constructing recombinant F-defective Sendai virus for preventing and treating human diseases in the form of vaccines and vectors for gene therapy. |
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