高效液相色谱法测定复方四维女贞子胶囊中牛磺酸的含量

目的建立复方四维女贞子胶囊中牛磺酸的质量控制方法。方法采用C18（250．0minx4．6mm，5μm）色谱柱；流动相：乙腈-0．1mol／L醋酸钠溶液（合0.1％5-乙胺，用醋酸调pH值至6．0）；流速1．0mL／min；检测波长254nm；柱温35℃。结果牛磺酸在0．1568-1．5680Ixg时，与峰面积的线性方程为Y=3×10^6-107537【相关系数（r）=0．9999]，回收率为98．72％，相对标准偏差（RSD）为0．59％（n=9）。结论高效液相色谱法简便、快速、准确，能有效控制复方四维女贞子胶囊中牛磺酸的质量。

Determination of taruine in compound tetravitamins and glossy capsules by HPLC

Objective To establish a method for the determination of taruine in compound tetravitamins and glossy capsules by high performance liquid chromatography（HPLC）. Methods Chromatographic analysis was performed on chromatographic column of C is （250.0 mm×4.6 mm, 5 μm） with mobile phase of acetonitrile -0.1 mol/L sodium acetate solution （contain 0.1% triethy- lamine, adjusted to pH=6.0 with acetic acid）. The flow rate was ] .0 mL/min with the detection wavelength at 254nm and the colum temperature at 35 ~C. Results The method showed a good linearity：Y=3×10^6X-107 537 [coefficient of association （r）=0.999 9] in the concentration range of 0.156 8-1.568 0 μg for taruine. The recovery rate was 98.72%, and relative standard deviation （RSD） was 0.59%（n=9）. Conclusion The HPLC is simple,rapid and accurate,and it can be used effectively for the quality control of taruine in compound tetravitamins and glossy capsules.