人乳头瘤病毒基因亚型检测在子宫颈疾病诊断中的意义

目的: 探讨不同人乳头瘤病毒（human papillomavirus,HPV）基因亚型的感染情况及高危型HPV亚型感染与子宫颈疾病间的关系。方法: 选取我院妇科门诊就诊的1 669例患者行HPV基因分型检测,HPV检测结果为阳性的患者在知情同意后行阴道镜检查,对镜下表现可疑的患者进一步行子宫颈组织活检。结果: 1 669例患者中HPV阳性567例,总HPV阳性率为33.97%,HPV亚型感染率前5位由高到低依次为HPV-16（9.05%）、HPV-52（7.85%）、HPV-58（6.29%）、HPV-CP8304（5.81%）、HPV-53（3.71%）。不同年龄段中,≤30岁者为HPV感染的高峰年龄段。在567例HPV阳性的患者中,高危型HPV亚型感染患者共454例,占总HPV感染者的80.07%。病灶组织活检结果为高级别鳞状上皮内病变(high-grade squamous intraepithelial lesion, HSIL)以上的患者中,高危型HPV亚型感染的前5位由高到低依次为HPV-16（58例）、HPV-52（26例）、HPV-58（25例）、HPV-18（10例）、HPV-31（6例）。结论: HPV感染人群高峰有年轻化趋势,HPV基因分型检测在子宫颈疾病诊断中具有重要价值,其中HPV-16、HPV-52、HPV-58、HPV-18、HPV-31与子宫颈癌的关系密切。

Significance of HPV genotype detection in diagnosis of cervical lesion

Objective: To investigate the infection of different genotypes of papillomavirus (HPV) and the relationship between high-risk HPV genotype and cervical lesion in females. Methods: HPV genotype detection was performed in 1 669 subjects. Colposcopy was conducted in positive cases after informed consent was obtained and biopsy was performed in suspected cases under colposcopy. Results: Five hundred and sixty-seven cases of the 1 669 subjects were HPV positive, with a positive rate of 33.97% (567/1 669). The five leading frequencies of HPV genotype were HPV-16 (9.05%), HPV-52 (7.85%), HPV-58 (6.29%), HPV-CP8304 (5.81%), HPV-53 (3.71%). Women ≤30 were at the peak of HPV infection when compared with other ages. Four hundred and fifty-four cases of 567 HPV positive subjects were infected with high-risk HPV genotype. The five leading frequencies of high-risk HPV infection with high grade squamous intraepithelial lesion (HSIL) in biopsy were HPV-16 (58 cases), HPV-52 (26 cases), HPV-58 (25 cases), HPV-18 (25 cases), HPV-31 (6 cases). Conclusions: The peak age for HPV infection is getting younger, and HPV genotype detection has significant value in the diagnosis of cervical lesion, especially HPV-16, HPV-52, HPV-58, HPV-18, HPV-31, they are closely associated with cervical cancer.