转染hEndostatin基因对CNE2细胞株裸鼠移植瘤生长的影响

背景与目的：肿瘤的生长、转移是血管生成依赖性的,血管生成抑制剂有望通过抑制肿瘤血管生成及诱导肿瘤细胞凋亡,有效地抑制肿瘤的生长和转移。本文旨在探讨转染人内皮抑素基因hEndostatin对人鼻咽癌细胞株CNE2裸鼠移植瘤生长的影响。方法：采用脂质体介导法,分别将pBlast-hIL-hEndostatin、pBlast-hEndostatin和pBlast-MCS质粒导入人鼻咽癌细胞株CNE2,MTT法在体外检测转导细胞表达的hEndostatin的活性,并在体内实验中观察转导的CNE2细胞在裸鼠上的致瘤性的差异。结果：转染pBlast-hIL-hEndostatin的CNE2细胞的上清能显著抑制血管内皮细胞ECV304的生长,其在裸鼠体内形成的瘤组织重量和体积均显著低于对照组（P＜0．01）,生长速度明显慢于对照组细胞。结论：转染hEndostatin基因能有效抑制CNE2细胞在裸鼠体内的生长。

Effect of transfection of hEndostatin gene on CNE2 cell xenograft growth in nude mice

BACKGROUND & OBJECTIVE:Tumor angiogenesis play an important role in growth and metastasis of cancer. Angiogenesis inhibitors induce apoptosis in cancer by inhibiting tumor angiogenesis and have strong inhibiting effect on both growth and metastasis of cancer. This study was designed to explore the effect of transfection of human endostatin gene on nasopharyngeal carcinoma CNE2 cells xenograft growth in nude mice. METHODS:The plasmids (pBlast hIL hEndostatin, pBlast hEndostatin, and pBlast MCS) were transfected and lipofectin mediated into the CNE2 cell line. The biological activity of secreted hEndostain from gene transferred cell lines was determined using MTT method in vitro. Then the transfected CNE2 cells were injected into the nude mice and tumorigenicity of CNE2 was observed in vivo. RESULTS:The supernatant of CNE2 cell transfected with pBlast hIL hEndostatin effectively inhibited the growth of endothelial cell (ECV304). The volume and the weight of tumor in pBlast hIL hEndostatin transfecting cells group were less than those in control group (P< 0 01). The growth speed of tumor in pBlast hIL hEndostatin transfecting cells group was slower than that in control group. CONCLUSION:Transfection of hEndostatin gene could inhibit CNE2 cell growth in nude mice.