多种转移潜能不同的人前列腺癌细胞“上皮细胞间质转化态”特性鉴定及其意义

目的:对多种转移潜能不同的人前列腺癌细胞“上皮细胞间质转化态”(EMT)特性进行鉴定,并从粘附因素和细胞骨架蛋白角度分析其骨转移潜能获得的分子机制。方法:用W estern印迹法鉴定LNCaP及其亚细胞系C4、C4-2和ArCaP亚细胞系IF11、IA8,以及PC-3、Du145等细胞中上皮型钙粘素(E-cadherin)、神经型钙粘素(N-cadherin)和波形纤维蛋白(V im entin)的表达差异情况,并分析其在前列腺癌转移过程中的作用。结果:E-cad-herin在PC-3、LNCaP、C4、C4-2中表达较高,但在Du145、IF11、IA8中表达极低;而V im entin的表达情况恰恰与E-cadherin相反;N-cadherin在IF11、IA8细胞中呈现显著的高表达状态。结论:转移潜能不同的人前列腺癌细胞株之间存在EMT表型的表达差异,其中PC-3、LNCaP、C4、C4-2是未发生EMT改变的细胞,Du145、IF11、IA8却是EMT化的细胞。EMT表型差异蛋白在解释前列腺癌转移机制方面占据着重要地位。

Expression of "Epithelial-mesenchymal Transition" Associated Proteins in Prostate Cancer Cell Lines with Different Metastatic Potentials and Its Significance

Objective: To determine the characteristics of epithelial-mesenchymal transition(EMT) in different human prostate cancer cell lines and explore the molecular mechanisms of bone metastatic potentials. Methods: Expressions of E-cadherin,N-cadherin and Vimentin in several prostate cancer cell lines(LNCaP,C4,C4-2,IF11,IA8,Du145 and PC-3) with different metastatic potentials were detected by Western blotting.Results: There was remarkable difference in the expressions of E-cadherin,N-cadherin and Vimentin between these cell lines.As one of the adhesion associated proteins,E-cadherin was detected with high expression in LNCaP,C4,C4-2 and PC-3,whereas with a low expression in IF11,IA8 and Du145.However,as one of the mesenchymal proteins,N-cadherin was shown to be completely different from Vimentin expression profile in these cell lines. Conclusion: There is actual difference in the EMT phenotypes among cell lines with different metastatic potentials.LNCaP,C4,C4-2 and PC-3 are cells without EMT change,while IF11,IA8 and Du145 are positive for EMT.The expressions of EMT associated proteins play important roles in promoting and repressing the metastasis of prostate cancer.