用荧光原位杂交从母血中检测胎儿细胞

目的 从母血中分离胎儿细胞并确定其来自胎儿。方法 从孕早、中期各２０名、分娩后１５名母血中富集并分离有核细胞。用Ｙ特异性探针（ＰＹ３．４）行荧光原位杂交，从中识别胎儿细胞。结果 孕早、中期孕妇各怀１５名男胎。阳性细胞比例是１：６５２８．０及１：２７３．８。与同期１０名女胎阳性细胞相比，差别有高度显著性。分娩１周内的３名，阳必民孕中期的差别没有显著性。分娩３个月后的阳性率与比，差别有高度显著性。分娩

Detection of fetal cells in maternal blood by fluorescence in situ hybridization

OBJECTIVE: To isolate fetal nucleated cells from maternal blood and determine its fetal origin. METHODS: Enrichment and isolation of nucleated cells in maternal blood from 20 samples in the first trimester of pregnancy, 20 samples in the mid-trimester of pregnancy, and 15 samples after delivery. Fluorescence in situ hybridization was performed using Y specific probe PY3.4 to identify fetal cells. RESULTS: Fifteen women in the first trimester and 15 women in the mid-trimester of pregnancy carried male fetuses. The rates of positive cells were 1:6528.0 and 1:2783.8 respectively, and there was a highly significant difference when these rates were compared with the positive cells rate of the 10 female fetuses in the same trimesters. No significant difference in positive cells rate was found between 1 week after delivery and the mid-trimester of pregnancy, nor was it found between 3 months after delivery and the 10 samples of female fetuses in the same time. CONCLUSION: These data suggest that fetal cells can be detected as early as from 50 days of gestation in maternal blood, in the meantime, the rate of fetal cells will increase with gestational age. One week after delivery, fetal cells still exist, and 3 months after delivery, the fetal cells will no longer be detected.