Sepharose 4B免疫吸附法制备红细胞试剂血清的研究

本文介绍采用 ABH 物质与 Sepharose 4B 偶联,吸收含非 ABO 抗体试剂血清中的抗-A 或抗-B。含 ABO 抗体及抗-D(11),-C(5),-E(5),-c(6),-e(4),-M(1),-N(1),-s(1),-k(1),-Kpb(1),-Fy~a(1),-Jsb(1)的血清以本法吸收后,再以 ABO 不合而无相应非 ABO 抗原的细胞及 O 型配组红细胞试验。结果:ABO 抗体均被吸收而非 ABO 抗体均得以保留;非 ABO 抗体不产生假阳性反应;反复吸收不引起非 ABO 抗体效价降低。本文还讨论了本法的优点及影响吸收效果的因素。

Study of Preparing the Red Cell Reagent Serum by Sepharose 4B Immunoadsorption

The absorption of anti-A and anti-B in reagent sera containing non-ABO antibodies,performed by coupling the ABH substance to se- pharose 4B,was introduced.The sera containing both ABO antibodies and anti-D(11),-C(5),-E(5),-c(6), -e(4),-M(1),-N(1),-s(1),-K(1), -Kp~b(1),-Fy~a(1),-Js~b(1)absorbed by this method,were tested then by the ABO-incompatible cells lacking relevant non-ABO antigens,and the panel red cells(O).As a result,all the ABO antibodies were absorbed exclusively,but all the non-ABO antibodies were preserved fullyThe non-ABO antibodies can't give the false positive reaction and their titer can't decrease after repeated absorp- tion.The advantages of this method and the factors that have influeced on effectiveness of the absorption were discussed.