Genetic variability of East African cassava mosaic Cameroon virus under field and controlled environment conditions |
| |
Authors: | Fondong Vincent N Chen Kegui |
| |
Affiliation: | Delaware State University, 1200 North DuPont Highway, Dover, DE 19901, USA Department of Biological Sciences, Delaware State University, 1200 North DuPont Highway, Dover, DE 19901, USA |
| |
Abstract: | Cassava geminiviruses occur in all cassava growing areas of Africa and are considered to be the most damaging vector-borne plant pathogens. At least seven species of these viruses have been identified. We investigated genetic variation in East African cassava mosaic cassava Cameroon virus (EACMCV) from naturally infected cassava and from experimentally infected Nicotiana benthamiana. Results showed that the populations of EACMCV in cassava and in N. benthamiana were genetically heterogeneous. Mutation frequencies in the order of 10−4, comparable to that reported for plant RNA viruses, were observed in both hosts. We also produced an EACMCV mutant that induces reversion and second site mutations, thus suggesting that a high mutation frequency facilitates the maintenance of genome structure and function. This is direct experimental evidence showing that cassava geminiviruses exhibit a high mutation frequency and that a single clone quickly transforms into a collection of mutant sequences upon introduction into the host. |
| |
Keywords: | Cassava geminivirus Genetic variability Mutation frequency Reversion |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|