Structural biology of retroviral DNA integration |
| |
Authors: | Li Xiang Krishnan Lavanya Cherepanov Peter Engelman Alan |
| |
Affiliation: | a Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA 02115, USAb Division of Infectious Diseases, Imperial College London, London W2 1PG, UK |
| |
Abstract: | Three-dimensional macromolecular structures shed critical light on biological mechanism and facilitate development of small molecule inhibitors. Clinical success of raltegravir, a potent inhibitor of HIV-1 integrase, demonstrated the utility of this viral DNA recombinase as an antiviral target. A variety of partial integrase structures reported in the past 16 years have been instrumental and very informative to the field. Nonetheless, because integrase protein fragments are unable to functionally engage the viral DNA substrate critical for strand transfer inhibitor binding, the early structures did little to materially impact drug development efforts. However, recent results based on prototype foamy virus integrase have fully reversed this trend, as a number of X-ray crystal structures of active integrase-DNA complexes revealed key mechanistic details and moreover established the foundation of HIV-1 integrase strand transfer inhibitor action. In this review we discuss the landmarks in the progress of integrase structural biology during the past 17 years. |
| |
Keywords: | HIV-1 Prototype foamy virus Integrase Integration AIDS Integrase strand transfer inhibitor Raltegravir Structural biology X-ray crystallography |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|