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Hepatoprotective effect of prolactin and cysteine contained in perfusion and preservation solutions on porcine liver stored in simple hypothermia |
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| Authors: | Ryszka F Dolińska B Caban A Ostróżka-Cieślik A Budziński G Krzysztofik M Oczkowicz G Cierpka L |
| Institution: | a “Biochefa” Pharmaceutical Research and Production Plant, Sosnowiec, Poland b Department of Applied Pharmacy, Medical University of Silesia, Sosnowiec, Poland c Department of General Vascular and Transplant Surgery, Medical University of Silesia, Katowice, Poland |
| Abstract: | IntroductionThe aim of our study was to determine the results of histidine-tryptophan-ketoglutarate (HTK) solutions modified by the addition of the antioxidant cysteine (Cys), and of prolactin (PRL) on storage of isolated porcine livers.MethodsWe measured in the media of isolated livers stored for 24 hours in HTK (control group) or modified HTK + Cys (0.3 mmol/L) + PRL (3 IU/L study group) the amounts of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactic acid as well as Ca (II), Mg (II), Na (I) and K (I) ions during a 30-minute perfusion after 24 hours of storage.ResultsAll tested markers were released more slowly into HTK + Cys + PRL with less release of K(I) and Mg(II) and greater of Na(I) and Ca(II) ions.ConclusionsAddition of the Cys and PRL to HTK positively affected 24-hour storage of isolated livers. |
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