Cowpox virus induces interleukin-10 both in vitro and in vivo |
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Authors: | Spesock April H Barefoot Brice E Ray Caroline A Kenan Daniel J Gunn Michael D Ramsburg Elizabeth A Pickup David J |
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Affiliation: | a Department of Molecular Genetics and Microbiology, Box 3020, Duke University Medical Center, Durham, NC 27710, USAb Duke University Human Vaccine Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USAc Department of Pathology, Duke University Medical Center, Durham, NC 27710, USAd Department of Medicine, and Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA |
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Abstract: | Cowpox virus infection induces interleukin-10 (IL-10) production from mouse bone marrow-derived dendritic cells (BMDCs) or cells of the mouse macrophage line (RAW264.7) at about 1800 pg/ml, whereas infections with vaccinia virus (strains WR or MVA) induced much less IL-10. Similarly, in vivo, IL-10 levels in bronchoalveolar lavage fluids of mice infected with cowpox virus were significantly higher than those after vaccinia virus infection. However, after intranasal cowpox virus infection, although dendritic and T-cell accumulations in the lungs of IL-10 deficient mice were greater than those in wild-type mice, weight-loss and viral burdens were not significantly different. IL-10 deficient mice were more susceptible than wild-type mice to re-infection with cowpox virus even though titers of neutralizing antibodies and virus-specific CD8 T cells were similar between IL-10 deficient and wild-type mice. Greater bronchopneumonia in IL-10 deficient mice than wild-type mice suggests that IL-10 contributes to the suppression of immunopathology in the lungs. |
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Keywords: | Interleukin-10 Cowpox Vaccinia Dendritic cell Inflammation Bronchopneumonia Rechallenge Immunopathology Lung |
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