Computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality |
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| Authors: | Robinson Christopher M Singh Gurdeep Henquell Cécile Walsh Michael P Peigue-Lafeuille Hélène Seto Donald Jones Morris S Dyer David W Chodosh James |
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| Affiliation: | a Howe Laboratory, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, 243 Charles Street, Boston, MA 02114, USAb CHU Clermont-Ferrand, Laboratoire de Virologie, Hôpital Gabriel Montpied, France, Université d'Auvergne, EA-3843, Francec Department of Bioinformatics and Computational Biology, George Mason University, 10900 University Boulevard, MSN 5B3, Manassas, VA 20110, USAd Clinical Investigation Facility, David Grant USAF Medical Center, 101 Bodin Circle, Travis AFB, Fairfield, CA 94535, USAe Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 975 N.E. 10th, Oklahoma City, OK 73104, USA |
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| Abstract: | Adenoviral infections are typically acute, self-limiting, and not associated with death. However, we present the genomic and bioinformatics analysis of a novel recombinant human adenovirus (HAdV-D56) isolated in France that caused a rare neonatal fatality, and keratoconjunctivitis in three health care workers who cared for the neonate. Whole genome alignments revealed the expected diversity in the penton base, hexon, E3, and fiber coding regions, and provided evidence for extensive recombination. Bootscan analysis confirmed recombination between HAdV-D9, HAdV-D26, HAdV-D15, and HAdV-D29 in the penton base and hexon proteins, centered around hypervariable loops within the putative proteins. Protein structure analysis of the fiber coding region revealed similarity with HAdV-D8, HAdV-D9, and HAdV-D53, possibly accounting for the ocular tropism of the virus. Based on these data, this virus appears to be a new HAdV-D type (HAdV-D56), underscoring the importance of recombination events in human adenovirus evolution and the emergence of new adenovirus pathogens. |
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| Keywords: | Adenovirus Viral genomics Viral recombination Viral evolution Viral bioinformatics |
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