| 天然牛磺酸对肝纤维化大鼠TGF-β1/Smad信号通路的影响 |
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| 引用本文： | 邓 鑫,梁 健,黄 彬,李益忠,覃黎葵,张锡流.天然牛磺酸对肝纤维化大鼠TGF-β1/Smad信号通路的影响[J].大连医科大学学报,2007,29(4):336-339. |
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| 作者姓名： | 邓 鑫 梁 健 黄 彬 李益忠 覃黎葵 张锡流 |
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| 作者单位： | 1. 广西中医学院附属瑞康医院,肝胆内科,广西,南宁,530011
2. 广西中医学院第-附属医院,病理科,广西,南宁,530023 |
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| 摘 要： | 目的]探讨天然牛磺酸对肝纤维化大鼠TGF-β1/Smad信号通路的影响.方法]Wistar大鼠随机分成对照组、模型组、天然牛磺酸治疗组,采用CCL4诱导肝纤维化模型.酶联免疫吸附法(EuSA)测定血清TGF-β1含量;免疫组织化学法检测肝组织TGF-β1、Smad3、Smad7蛋白表达;RT-PCR检测肝组织TGF-β1、TβRⅠ和TβRⅡmRNA表达;分别用HE染色和Masson染色.肝组织观察病理组织变化.结果]治疗组血清TGF-β1含量为(1.65±0.32)μg/L,显著低于模型组(3.93±0.32)μg/L,P＜0.01;治疗组肝组织TGF-β1,Smad3蛋白表达分别为(4.5±0.6)%、(2.5±0.8)%,显著低于模型组(7.5±1.2)%、(3.9±0.8)%,P＜0.05,Smad7为(6.2±1.3)%,显著高于模型组(1.3±0.2)%,P＜0.01;治疗组肝组织TGF-β1,TβRⅠ和TβRⅡmRNA表达分别为0.83±0.21、0.45±0.16、0.63±0.15,显著低于模型组1.62±0.45、0.93±0.24、1.25±0.45,P＜0.01;治疗组肝纤维化分期积分为2.07±0.31,显著低于模型组3.18±0.42,P＜0.01.结论]天然牛磺酸能抑制TGF-β1及其受体和Smad3的表达,上调Smad7的表达,具有抑制肝纤维化的作用.
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| 关 键 词： | 牛磺酸 肝纤维化 转化生长因子-β1 Smad |
| 文章编号： | 1671-7295（2007）04-0336-04 |
| 收稿时间： | 2006-11-21 |
| 修稿时间： | 2007-03-19 |
Effects of natural taurine on TGF-β1/Smad singaling pathway in rats of hepatic fibrosis |
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| DENG Xin,LIANG Jian,HUANG Bin,LI Yi-zhong,QIN Li-kui and ZHANG Xi-liu.Effects of natural taurine on TGF-β1/Smad singaling pathway in rats of hepatic fibrosis[J].Journal of Dalian Medical University,2007,29(4):336-339. |
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| Authors: | DENG Xin LIANG Jian HUANG Bin LI Yi-zhong QIN Li-kui and ZHANG Xi-liu |
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| Institution: | The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China;;The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China;;The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China;;The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China;;The Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China;;The First Affiliated Hospital of Guangxi Traditional Chinese Medical College,Nanning 530011,China |
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