骨髓基质细胞移植对大鼠脊髓损伤后环氧化酶-2和血管内皮生长因子表达的影响

目的：观察骨髓基质细胞（bone marrow stromal cells，BMSCs）移植对大鼠脊髓损伤后环氧化酶-2（cyclooxygenase-2， COX-2）和血管内皮生长因子（vascular endothelial growth factor，VEGF）蛋白表达的影响。方法大鼠随机分为3组：A为假手术组，B为细胞培养基（DMEM）对照组，C为BMSCs移植组。B组和C组大鼠进行脊髓压迫损伤模型制作。脊髓损伤后， B组和C组大鼠分别给予DMEM培养液或BMSCs脊髓损伤周围区注射。术后应用Western Blot方法检测COX-2、VEGF蛋白在损伤脊髓组织中的表达变化。结果 COX-2、VEGF蛋白在A组大鼠未损伤脊髓组织中均低水平表达。B组与A组相比，脊髓损伤后COX-2蛋白表达水平明显增加（P＜0.01），VEGF蛋白表达水平则短暂显著增加（P＜0.05）。C组与B组相比， COX-2和VEGF蛋白表达水平均显著增加。结论 BMSCs移植能够增加脊髓损伤后COX-2、VEGF蛋白的表达。

Effect of bone marrow stromal cells transplantation on expressions of cyclooxygenase-2 and vascular endothelial growth factor in rats following spinal cord injury

Objective To observe the effect of bone marrow stromal cells （BMSCs） transplantation on expression of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor （VEGF） protein in rats following spinal cord injury （SCI）. Methods Adult rats were randomly divided into group A （sham operation group）, group B （DMEM control group）, and group C （BMSCs transplantation group）. A rat spinal cord compression injury model was established. Rats in groups B and C were injected with BMSCs or DMEM after SCI. Expression of COX-2 and VEGF protein in rats following SCI was detected by Western blot. Results The expression level of COX-2 and VEGF protein was low in group A. The expression level of COX-2 and VEGF protein was significantly higher in group B than in group A and in group C than in group B （P〈0.01, P〈0.05）. Conclusion BMSCs transplantation can up-regulate the expression of COX-2 and VEGF protein in rats following SCI.