T辅助细胞亚型细胞相关因子在类风湿关节炎发病中的作用

目的探讨T辅助细胞(Th)1、Th2、Th17、调节性细胞(Treg)相关细胞因子在类风湿关节炎(RA)发病中的作用。方法选取71例RA患者作为研究对象,并根据DAS28评分,分为高、中、低活动组,采集试验对象及健康对照组(18名)外周血,采用微量样本多指标流式蛋白定量技术(CBA)和酶联免疫吸附试验(ELISA)方法检测白细胞介素(IL)-2、IL-4、IL-6、IL-10、IL-17A、干扰素(IFN)-γ、肿瘤坏死因子(TNF)-α和转化生长因子(TGF)-β8种细胞因子血清浓度。结果 RA患者IL-2、IL-4、IL-6、IL-10、IL-17A、IFN-γ、TNF-α血清浓度与健康对照组比较差异有统计学意义(P<0.01);TGF-β血清浓度与健康对照组比较差异无统计学意义(P=0.078);并且随着DAS28的不同其血清浓度也不同。结论推测Th1、Th2、Th17、Treg细胞的相关因子均参与了RA的致病过程,并且在不同的发病阶段发挥不同的作用。

The role of T-helper subtype cell related cytokines in the occurrence of rheumatoid arthritis

Objective To explore the the role of Th1, Th2, Th17 and Treg cells subtype cell related cytokines in occurrence of rheumatoid arthritis (RA). Methods Seventy-one RA patients were selected and divided into high, moderate and low activity groups according to DAS28 scoring. Thereafter, the peripheral blood (PB) was collected in the RA and healthy control groups and detected its serum concentrations of cytokines including IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF-α and TGF-β by using cytometeric bead array (CBA) and enzyme-linked immunosorbent assay (ELISA). Results Significant difference could be found in the serum concentrations of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α cytokines (P<0.01), but not in that of TGF-β cytokine (P=0.078) between RA patients and healthy control group. Furthermore, the serum concentration was different along with altered DAS28. Conclusion It could be indicated that the related cytokines of Th1, Th2, Th17 and Treg cells had participated into the pathogenesis of RA and exerted different effects in different occurrence stages.