Platelet characteristic antigens of CD34+ cells in cryopreserved cord blood: a study of platelet-derived microparticles in transplant processing |
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Authors: | Liu B Chen J S Cao M Gu S L Liao C Li D Z Zhong H Z |
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Affiliation: | Medical Experimental Centre, Guangzhou 12th People's Hospital, Guangzhou, China. binliu7@hotmail.com |
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Abstract: | BACKGROUND AND OBJECTIVES: In previous studies, we found that platelet microparticles (PMPs) bind to cord blood (CB) CD34+ cells and transfer adhesion molecules to them, which enhances their engraftment. Before applying this phenomenon in actual transplants, we investigated the effect of PMPs on cryopreserved CD34+ cells in CB. MATERIALS AND METHODS: We cryopreserved 18 CB units, then evaluated the binding of PMPs to CD34+ cells after thawing, by varying the expression of platelet characteristic antigens (CD41a, CD61, CD62P and CXCR4) on these cells. Adherence of the CD34+ cells, coated with freeze/thaw-induced PMPs, to endothelium and fibronectin was also studied, as were the effects of thrombin-induced PMPs from both fresh and preserved CB platelets. RESULTS: PMPs induced by freezing and thawing adhered less well to CD34+ cells than did those from fresh CB, and cells coated with these PMPs had poor adherence. However, thrombin-induced PMPs from both fresh and preserved CB platelets bound equally well to cryopreserved CD34+ cells and improved their adhesion properties. CONCLUSIONS: PMPs could be a useful tool for enhancing engraftment after CB transplantation. |
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Keywords: | CD34+ cells cord blood transplantation cryopreservation platelet microparticles transplantation |
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