| 高效液相色谱法测定藿香正气胶囊中橙皮苷的含量 |
| |
| 引用本文： | 王丽丽,张宝华,寇筱囡,王锐. 高效液相色谱法测定藿香正气胶囊中橙皮苷的含量[J]. 黑龙江医学, 2010, 34(6): 424-426. DOI: 10.3969/j.issn.1004-5775.2010.06.009 |
| |
| 作者姓名： | 王丽丽 张宝华 寇筱囡 王锐 |
| |
| 作者单位： | 大庆市龙南医院药剂科、齐齐哈尔医学院附属第五医院,黑龙江,大庆,163453;大庆油田总院集团油田总医院检验科,黑龙江,大庆,163453 |
| |
| 摘 要： | 目的建立高效液相色谱法测定藿香正气胶囊中橙皮苷的含量。方法采用高效液相色谱方法。色谱柱为十八烷基硅烷键合硅胶为填充剂〔DiamosilTM(钻石),5.0μm,200 mm×4.6 mm〕,流动相为甲醇-0.5%醋酸溶液(30∶70),检测波长为284 nm,流速为1.0 mL/min。结果橙皮苷在5.592～74.56μg/mL范围内呈良好的线性关系,回归方程为A=21 168.38C-8 066.67,R=0.999 99。回收率为99.32%。结论该方法简单、快速、准确,能消除干扰,可用于测定藿香正气胶囊中橙皮苷的含量。
|
| 关 键 词： | 橙皮苷 高效液相色谱法 含量 |
Determination Hesperidin Content of Huoxiangzhengqi Capsule by the Way of High Performance Liquid Chromatography |
| |
| Affiliation: | Wang Li-li,Zhang Bao-hua,Kou Xiao-nan,et al.(Department of Pharmacy Service,the Longnan Hospital of Daqing City of Heilongjiang Province,Daqing 163453,CHINA) |
| |
| Abstract: | Objective To establish high performance liquid chromatography（HPLC） and assay hesperidin content of Huoxiangzhengqi capsule.Methods To use the way of HAPC.Chromato bar is octadecylsilane chemically bonded silica as bulking agent（DiamosilTM,5.0 μm,200 mm×4.6 mm）,Mobile phase is methanol-0.5% acetic acid solution（30：70）,Detected wave length is 284nm,Flow rate is 1.0 mL/min.Results Hesperidin has satisfactory linear correlation between 5.592～74.56μg/mL.Regression Equation is A=21 168.38C-8 066.67,R=0.999 99.Coefficient of recovery is 99.32%. |
| |
| Keywords: | Hesperidin High performance liquid chromatography Content |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
