| 中低压制备色谱从枳实中制备柚皮苷与新橙皮苷对照品的研究 |
| |
| 引用本文： | 廖茂梁,尚海花,王 阳,张铁军. 中低压制备色谱从枳实中制备柚皮苷与新橙皮苷对照品的研究[J]. 医学教育探索, 2013, 44(10): 1271-1274 |
| |
| 作者姓名： | 廖茂梁 尚海花 王 阳 张铁军 |
| |
| 作者单位： | 1.天津药物研究院，天津 300193 2.天津中医药大学，天津 300193 |
| |
| 基金项目： | 国家科技支撑计划项目（2007BAI37B07） |
| |
| 摘 要： | 目的 建立从枳实中分离制备柚皮苷和新橙皮苷对照品的方法。方法 结合大孔树脂、中低压制备色谱分离制备枳实中柚皮苷和新橙皮苷单体。结果 分离制备的柚皮苷的质量分数达98.76%，新橙皮苷的质量分数达99.50%。结论 该方法可有效制备高纯度的柚皮苷和新橙皮苷，可用于制备中药定性、定量分析使用的对照品。
|
| 关 键 词： | 柚皮苷；新橙皮苷；枳实；对照品；中低压制备色谱 |
Preparation of naringin and neohesperidin reference substances from Citrus aurantium by medium-low-pressure preparative chromatography |
| |
| LIAO Mao-liang,SHANG Hai-hu,WANG Yang,ZHANG Tie-jun. Preparation of naringin and neohesperidin reference substances from Citrus aurantium by medium-low-pressure preparative chromatography[J]. Researches in Medical Education, 2013, 44(10): 1271-1274 |
| |
| Authors: | LIAO Mao-liang SHANG Hai-hu WANG Yang ZHANG Tie-jun |
| |
| Affiliation: | 1.Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 2.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
| |
| Abstract: | Objective To establish a separation method for naringin and neohesperidin reference substances from Citrus aurantium. Methods Naringin and neohesperidin monomers in C. aurantium were isolated and purified by macroporous resin and medium-low- pressure preparative chromatography. Results The contents of the prepared naringin and neohesperidin reached to 98.76% and 99.50%, respectively. Conclusion This method is effective for the preparation of naringin and neohesperidin with high purity. It could be used to prepare the reference substances for the qualitative and quantitative analyses of Chinese materia medica. |
| |
| Keywords: | naringin neohesperidin Citrus aurantium L. reference substance medium-low-pressure preparative chromatography |
|
| 点击此处可从《医学教育探索》浏览原始摘要信息 |
|
点击此处可从《医学教育探索》下载全文 |
