瑞香狼毒不定芽生长过程黄酮类和香豆素类成分累积研究

目的 研究瑞香狼毒Stellera chamaejasme不定芽生长过程黄酮类和香豆素类成分的累积规律。方法 采用组织培养筛选不定芽诱导和生长的最适培养基；分别通过分光光度法、高效液相色谱法分析不定芽生长过程总黄酮和3种香豆素类成分伞形花内酯、瑞香素和东茛菪内酯的动态累积。结果 不定芽诱导最适培养基为MS＋0.5 mg/L BA＋0.5 mg/L NAA，培养4周后，平均每个顶芽诱导35个不定芽；不定芽生长最适培养基为MS＋0.3 mg/L BA＋0.1 mg/L NAA，培养4周后，芽平均株高为2.3 cm；生长在最适培养基的不定芽，其总黄酮的累积量在第5周达到最高，为0.218 g；3种香豆素类成分的量在整个不定芽生长中均表现随培养时间而增加，到第5周，质量分数均达到最高值，但均不及野生根中的量，而且质量分数高低顺序也发生变化。结论 瑞香狼毒不定芽中黄酮类和香豆素类成分累积最高为培养后的第5周。

Accumulation of flavonoids and coumarins during adventitious bud growth in Stellera chamaejasme

Objective To investigate the accumulation of flavonoids and coumarins during the growth of adventitious buds in Stellera chamaejasme. Methods The optimal culture media for the induction and the growth of adventitious buds were screened through tissue culturing. The dynamic accumulation of the total flavonoids and three components of coumarin (umbelliferone, daphnetin, and scopoletin) was analyzed by visible spectrophotometry and high-performance liquid chromatography methods, respectively. Results The optimal condition for the induction of adventitious bud proliferation was MS + 0.5 mg/L BA + 0.5 mg/L NAA; After 4-week culture, the number of adventitious buds in every tip-bud was 35; The optimal condition for the growth of adventitious bud was MS + 0.3 mg/L BA + 0.1 mg/L NAA; After 4-week culture, the average length of buds was 2.3 cm; The amount of total flavonoids increased to the highest of 0.218 g after 5-week culture of the adventitious buds; The amount of the three components of coumarin increased with culturing time increasing during the growth of adventitious buds, and increase to the highest at week 5. But the contents of the components were lower than those in the wild roots, and the descending order had changed. Conclusion The accumulation of flavonoids and coumarins in adventitious buds of S. chamaejasme could reach to the highest at week 5 of culture.