| HPLC测定复方盐酸二甲双胍格列美脲胶囊中的格列美脲 |
| |
| 引用本文： | 白靖,曹亮,张倩,方瑜,曹德英. HPLC测定复方盐酸二甲双胍格列美脲胶囊中的格列美脲[J]. 华西药学杂志, 2012, 27(4): 438-440 |
| |
| 作者姓名： | 白靖 曹亮 张倩 方瑜 曹德英 |
| |
| 作者单位： | 1. 河北医科大学药学院,河北石家庄,050017
2. 石药集团,河北石家庄,050017 |
| |
| 摘 要： | 目的用HPLC法测定复方盐酸二甲双胍格列美脲胶囊中格列美脲的含量。方法采用Diamonsil C18色谱柱(250mm×4.6 mm,5μm),流动相为0.05 mol·L-1甲酸铵缓冲液(pH4)-乙腈(40∶60),检测波长228 nm,流速1.0 mL·min-1。结果 1.014～20.280μg·mL-1格列美脲与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.5%,RSD=1.0%。结论所用方法简便、准确性高、专属性强,且盐酸二甲双胍对格列美脲的测定无干扰,可用于该类复方制剂的质量控制。
|
| 关 键 词： | 盐酸二甲双胍 格列美脲 高效液相色谱法 含量 |
Determination of glimepiride in Complex metformin hydrochloride-glimepiride capsules |
| |
| BAI Jing , CAO Liang , ZHANG Qian , FANG Yu , CAO De-ying. Determination of glimepiride in Complex metformin hydrochloride-glimepiride capsules[J]. West China Journal of Pharmaceutical Sciences, 2012, 27(4): 438-440 |
| |
| Authors: | BAI Jing CAO Liang ZHANG Qian FANG Yu CAO De-ying |
| |
| Affiliation: | 1(1.College of Pharmaceutical Science,Hebei Medical University,Shijiazhuang,Hebei,050017 P.R.China;2.CSPC Pharmaceutical Group Limited,Shijiazhuang,Hebei,050017 P.R.China) |
| |
| Abstract: | OBJECTIVE To establish an HPLC method for content determination of glimepiride in Complex metformin hydrochloride-glimepiride capsules.METHODS The chromatographic separation was performed on a Diamonsil C18 column（250 mm×4.6 mm,5 μm）.The mobile phase consisted of 0.05 mol·L-1 ammonium formate（pH4）and acetonitrile（40:60） at a flow rate of 1.0 mL·min-1,with detection wavelength of 280 nm.RESULTS The calibration curves showed a good linearity within the range of 1.014-20.280 μg·mL-1 for glimepiride（r=0.9999）.The mean recovery was 99.5% with the RSD of 1.0 %.CONCLUSION It is a simple,accurate and specific method with no interference between metformin hydrochloride and glimepiride.It should be a referred method for determination of the content of glimepiride in the complex formulations containing glimepiride. |
| |
| Keywords: | Metformin hydrochloride Glimepiride HPLC Content |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
