巴戟天不同炮制品的高效薄层色谱指纹图谱研究

目的以巴戟天药材生品为基础,建立巴戟天不同炮制品的高效薄层色谱(HPTLC)指纹图谱,并比较分析生品的指纹图谱。方法硅胶GF254高效预制薄层板,乙酸乙酯部位以石油醚-乙酸乙酯(5∶5.5)为展开剂,上行展开8 cm,10%硫酸乙醇溶液显色;正丁醇部位以正丁醇-冰醋酸-水(4∶1∶5)上层溶液为展开剂,上行展开8 cm,α-萘酚浓硫酸试剂显色。显色后置紫外光灯365 nm或自然光下检视,得薄层色谱指纹图谱,并进行相关分析。结果巴戟天生品的薄层荧光色谱指纹图谱与巴戟肉(蒸制)、盐巴戟天、制巴戟天(甘草制)图谱比较分析,彼此具有明显的区别,可用于生品与巴戟天炮制品的鉴别。结论通过HPTLC指纹图谱考察,生品巴戟天与巴戟肉、盐巴戟天、制巴戟天的区别较大,应分别制定质量评价的标准。

Study on the differences of Morinda officinalis and its processed products by HPTLC fingerprint chromatogram

OBJECTIVE The High-performance thin-layer(HPTLC) chromatographic fingerprint of Morinda officinalis and its processed products was established and their chromatograms were evaluated.METHODS The chromatographic conditions were as follows:pre-coated HPTLC GF254 silica-gel plate stationary phase,two parts of ethyl acetate and n-butyl alcohol were petroleum ether-ethyl acetate(5:5.5)and n-butyl alcohol-glacial acetic acid-water(4:1:5) as mobile phase(solvernt system),respectively.After developing about 8 cm and removal of the plate,dry it in air,10% sulfuric acid alcoholic and α-naphthol sulfuric acid solution as derivatization reagents.The common pattern of HPTLC fingerprints of Morinda officinalis and its processed products were analyzed under the conditions of UV 365 nm or natural light.RESULTS There were significant difference between the common patterns of Morinda officinalis and its precessed products by comparison with the HPTLC images.CONCLUSION The study shows that raw,steam,salt-processed,glycyrrhiza-processed products of Morinda officinalis can be distinguished by comparison with the HPTLC images.There are significant differences in their constituents.Thus it is suggested that quality control of Morinda officinalis and its processed products should be evaluated by means of HPTLC and other methods.