CYP2D6*10多态性与肺癌易感性

目的 探讨CYP2D6*10等位基因C188T和G4268C位点单核苷酸多态性与肺癌遗传易感性的关系.方法 采用PCR-RFLP法检测118例肺癌患者和按性别、年龄频数匹配的118名正常对照者CYP2D6基因C188T和G4268C位点的单核苷酸多态性,Logistic回归分析各基因型与肺癌发病危险的关系以及基因多态与吸烟在肺癌发生中的联合作用.结果 非T188/T(C188/C C188/T)基因型和非CA268/C(G4268/G G4268/C)基因型与肺癌均有中等强度关联.按照吸烟情况进行分层分析后发现,不吸烟者、轻度吸烟者中携带非T188/T基因型或非CA268/C基因型的个体患肺癌风险增高.但是CYP2D6非T188/T基因型或非CA268/C基因型与吸烟在肺癌的发生中均不存在交互作用,CYP2D6C188T、G4268C两位点之间也不存在交互作用.结论 T188/T和C4268/C基因型在不吸烟者和轻度吸烟者中可能作为保护因素而降低肺癌易感性.

CYP2D6*10 Polymorphisms and Lung Cancer Susceptibility

ObjectiveTo analyze the correlation between the polymorphism of CYP2D6*10 allele and the risk of lung cancer. Methods A case-control study was conducted among 118 lung cancer patients and 118 control subjects (matched for sex and age) to investigate the role of the single nucleotide polymorphism of C188T and G4268C in lung cancer using PCR-RFLP to identify the genotypes. Logistic regression was taken to analyze the relationship between genetic polymorphism and lung cancer susceptibility and evaluate the combined effect of genotypes and smoking. Results The non-T188/T genotype (including C188/C and C188/T genotype) or non-C4268/C genotype (including G4268/G and G4268/C genotype) was moderately correlated with lung cancer. After stratification analysis according to smoking, it revealed that non-T188/T or non-C4268/C genotype was associated with significantly increased risk in non-smokers and light-smokers subgroups; however, there was no interaction between non-T188/T or non-C4268/C genotype and smoking on susceptibility to lung cancer, neither between the two genotypes. Conclusion T188/T or C4268/C genotype, as protective factors in non-smokers or light-smokers subgroups, may help decrease the susceptibility to lung cancer in these subjects.