针刺对背根节神经元GDNF表达的影响

目的 研究部分去背根及针刺对背根神经节神经元胶质细胞源性神经营养因子（GDNF）表达的影响。方法 制作猫备用背根模型并针刺备用根支配区的两组穴位，足三里和悬钟，伏兔和三阴交。针刺7d后取备用背根节用GDNF抗血清（1：1000）行免疫组织化学染色。分别计数各组大，中，小阳性神经元的数值。结果 非手术组GDNF阳性神经元数量为55％。其中大神经元为25％，中神经元为14％，小神经元为16％，术后非针刺组，阳性神经元总数为35％，较非手术组明显下降（P＜0．05），主要为大神经元（5％）减少，而阳性少（13％），小神经元（17％）数量变化不明显，但染色明显加深。针刺组GDNF阳性神经元数量又明显增加至90％，其中阳性大神经元28％，中神经元17％，小神经元45％。结论 背根节GDNF阳性大神经元数量在去部分背根术后7d显著下降，而针刺可逆转这种变化。GDNF阳性中，小神经元数量在术后7d并无明显变化，但针刺后表达显著增加。

Expression of GDNF in Dorsal Root Ganglion after Partial Dorsal Root Rhizotomy and Acupuncture in Spared Root

OBJECTIVE: Investigating the expression of glial cell line-derived neurotrophic factor (GDNF) in dorsal root ganglion after partial dorsal root rhizotomy and acupuncture in spared root. METHODS: Fifteen adult cats were divided into three groups. Two groups were subjected to unilateral spared root rhizotomy (L1-L5, L7-S2 DRGs were sectioned, L6 DRG was spared). In one of these operating groups, two sets of acupoints (Zusanli and Xuanzhong; Futu and Sanyinjiao) located in the distribution area of spinal nerve L6 were electro-stimulated alternatively 30 min everyday by electro-needling. The other operation group was not treated with acupuncture. On the 7th day after partial dorsal rhizotomy and acupuncture, the animals of these two groups and another group (intact animals as reference group) were sacrificed. The DRGs (L6) were taken and made into frozen sections and were stained under the same condition using specific GDNF antibody (1:1000) by ABC method. The GDNF positive large, medium and small sized neurons in DRG were counted. RESULTS: In intact animals, about 55% neurons, including large sized neurons (25%) and a few small (16%) and medium sized neurons (14%), were stained. In contrast, at 7 days after partial dorsal root rhizotomy, the numbers of positive neurons decreased greatly (35%) more than that of reference group (P < 0.05). However, following acupuncture, about 90% neurons, including large (28%) medium (17%) and small (45%) neurons, came to be stained again. CONCLUSION: The number of GDNF positive large neurons decreased greatly at 7 days following partial dorsal root rhizotomy, but increased greatly after acupuncture. In addition, the number of GDNF positive small neurons increased greatly after acupuncture (P < 0.05).