| 人乳牙牙髓干细胞的体外培养观察 |
| |
| 引用本文： | 陆家瑜,刘翳文,华丽,周文锐,邹德荣.人乳牙牙髓干细胞的体外培养观察[J].口腔颌面外科杂志,2007,17(1):25-28. |
| |
| 作者姓名： | 陆家瑜 刘翳文 华丽 周文锐 邹德荣 |
| |
| 作者单位： | 上海交通大学附属第六人民医院口腔科,上海,200233 |
| |
| 摘 要： | 目的:体外分离、培养人乳牙牙髓干细胞。方法:采用酶消化法将人乳牙牙髓分离培养,以获得人乳牙牙髓干细胞。有限稀释法分离纯化,测定细胞克隆形成率,细胞计数法测生长曲线,细胞爬片行HE染色、碱性磷酸酶染色、抗波形蛋白(vimentin)免疫组化染色。结果:通过有限稀释法获得了克隆形成率为11～24个/103,细胞群体倍增时间为39.84h,HE染色细胞形态为梭形、细胞体小、胞核大的干细胞。且碱性磷酸酶染色阳性,抗波形蛋白(vimentin)免疫组化染色阳性。结论:人乳牙牙髓中可分离培养出牙髓干细胞。
|
| 关 键 词： | 乳牙 干细胞 分离 细胞培养 |
| 文章编号： | 1005-4979（2007）01-0025-04 |
| 收稿时间： | 2006-10-09 |
| 修稿时间： | 2006年10月9日 |
Human Dental Pulp Stem Cells from Deciduous Teeth Cultured in vitro |
| |
| LU Jia-yu,LIU Yi-wen,HUA Li,ZHOU Wen-rui,ZHOU De-rong.Human Dental Pulp Stem Cells from Deciduous Teeth Cultured in vitro[J].Chinese Journal of Oral and Maxillofacial Surgery,2007,17(1):25-28. |
| |
| Authors: | LU Jia-yu LIU Yi-wen HUA Li ZHOU Wen-rui ZHOU De-rong |
| |
| Abstract: | Objective: To isolate, culture and identify dental pulp stem cells from human deciduous teeth. Methods: Dental pulp stem cells from deciduous teeth were collected with enzyme digestion, isolated and purified by limited dilution. Examined the cells clone formation rate, measurement of cells growth curve by counting the number of cells, HE and alkaline phophatase(AKP)stain, and immunhistochemistry stain of vimentin. Result: Stem cells were obtained. The cells clone form rate was 11～24 clones/103 cells, cells group double time was 39.84 hours. AKP stain and immunhistochemistry stain of vimentin were both positive. Conclusion: Dental pulp stem cells can be collected from deciduous teeth successfully. |
| |
| Keywords: | deciduous teeth stem cells isolate cell cluture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
