| 千金子提取物抗肿瘤作用的实验研究 |
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| 引用本文： | 薛存宽,孔彩霞,黄晓桃,何学斌,刘志高. 千金子提取物抗肿瘤作用的实验研究[J]. 中国中西医结合杂志, 2004, 0(Z1) |
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| 作者姓名： | 薛存宽 孔彩霞 黄晓桃 何学斌 刘志高 |
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| 作者单位： | 华中科技大学同济医学院老年医药学研究所 华中科技大学同济医学院老年医药学研究所 武汉430077） 武汉430077） |
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| 摘 要： | 目的探索千金子的抗肿瘤作用。方法采用极性梯次提取与硅胶柱层析分离,并对其不同极性段的提取物和分离物进行了体内(小鼠移植性肿瘤)、外(噻唑蓝染色法)抗肿瘤活性研究。结果千金子的氯仿、丙酮段提取物对 K562,HepG_2和 U937细胞株具有抑瘤作用,对在体的 EAC、S180呈现出明显疗效;其石油醚、甲醇、水段提取物则基本上未显示有明显抗癌活性;该有效提取物的硅胶柱层析的多组分离物对K562,HL-60、Hela 细胞呈现出较强的细胞毒作用。结论除已知的千金子因子5外,千金子中还存在有多种细胞毒物质,其物质有抗肿瘤作用。
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| 关 键 词： | 千金子提取物 极性梯次 层析 抗肿瘤 噻唑蓝染色法 |
Experimental Study on Extract of Euphorbiae in Anti-tumor |
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| XUE Cun-kuan KONG Cai-xia HUANG Xiao-tao Institute of Senile Disease,Tongji Medical College,Huazhong University of Science and Technology,Wuhan. Experimental Study on Extract of Euphorbiae in Anti-tumor[J]. Chinese journal of integrated traditional and Western medicine, 2004, 0(Z1) |
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| Authors: | XUE Cun-kuan KONG Cai-xia HUANG Xiao-tao Institute of Senile Disease Tongji Medical College Huazhong University of Science Technology Wuhan |
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| Abstract: | Objective To explore the effect of extract of Euphorbiae in anti-tumor.Methods Seeds of Euphorbiae was ex- tracted by polar grade and isolated by silica gel column chromatography.The exytracts of different concentrations of polar grade and the isolators of chromatography were studied on the anti-tumor activity in vitro(by MTT method)and in vivo (grafting tumors in mice).Results The chloroform extract and the acetone extract from Euphorbiae had trmor inhibition effect on K562,HepG_2 and U937 cell line.Besides,they showed obvious therapeutic efficacy in EAC and S180 in vivo. However,the petroleum ether extract,methanol extract and distilled water extract from Euphorbiac showed no obvious anti- tumor activity basically.The multi-isolators of chromatography of effective extracts manifested stronger cytotoxicity on K562,HL-60 and Hela cell line.Conclusion Besides the known Euphorbiae factor 5,there exist many kinds of cytotoxic substance in Euphorbiae with the anti-tumor activity. |
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| Keywords: | Extract of Euphorbiae polar grade chromatography anti-tumor MTT method |
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