氯丙烯对神经细胞NO、MDA含量和NOS、SOD、GSH-PX活性的影响

探讨氯丙烯对神经细胞NO生成系统和抗氧化系统的影响。方法用NO、MDA、NOS、SOD、GSH-PH检测试剂盒研究了氯丙烯对鸡胚脑神经细胞NO、MDA含量和NOS、SOD、GSH-PX活性的影响。结果与对照组相比，随着氯丙烯浓度的增加，NO含量细胞内明显增加(P<0.01)，细胞外无显著差异；NOS活性细胞内中、高剂量组显著增加(P<0.05，P<0.01)，细胞外活性仅高剂量组显著增加(P<0.01)；细胞MDA含量明显降低，并呈明显的剂量-效应关系(r=-0.9693，P<0.05)；GSH-PX活性细胞内逐渐升高，呈明显的剂量-效应关系(r=0.9872，P<0.05)，细胞外仅高剂量组明显升高(P<0.01)；细胞内T-SOD和CuZn-SOD活性在3个剂量均明显下降(P<0.01)。结论氯丙烯可影响NO生成系统和细胞内抗氧化系统，这可能是氯丙烯引起细胞内Ca2+稳态失调的原因之一。

EFFECTS OF ALLYL CHLORIDEON NO, MDA CONTENTS AND NOS, SOD,GSH-PX ACTIVITIES IN NERVE CELLES

Objective:To research the poisioning mechanisms of allyl chloride and probe into its effects on NO sythesis system and antioxidation system in chicken embryo nerve cells.Methods:The contents of NO and MDA and the activities of NOS,SOD,GSH PX were done with related kits.Results:With allyl chloride increasing,the content of NO increased signifcantly in cells(P<0.01),but it appeared no different in medium.The activities of NOS increased significantly (P<0.01)both in nerve cells and medium.Cell MDA contents decreased significantly (P< 0.01 ) but GSH PX activity increased significantly both in nerve cells and in medium,(P< 0.01 ).T SOD and CuZn SOD activities decreased markedly (P<0.01).Conclusion:Allyl chloride can disturb NO synthesis and antioxidation in nerve cells.It might be one of the mechanisms of calcium unhomeostasis induced by allyl chloride.