喉癌p53基因突变与人乳头状瘤病毒感染的关系

目的探讨喉癌细胞ｐ５３基因突变与人乳头状瘤病毒（ＨＰＶ）感染的关系及其临床意义。方法应用星普旋转离心柱Ｂ溶液系统，结合聚合酶反应链多态性分析（ＰＣＲ－ＳＳＣＰ）及ＤＮＡ序列分析（ＰＣＲ－ＤＮＡＳｅｑｕｅｎｃｉｎｇ），对３６例喉癌组织新鲜标本进行ＨＰＶ定型、相对定量及ｐ５３基因第４至第８外显子突变检测。结果３６例喉癌标本中有１３例发生基因点突变或转码突变，其中１０例为ＨＰＶ１６／１８感染者，且与细胞内病毒含量呈正相关。２４例晚期喉癌（临床Ⅲ－Ⅳ期）有颈淋巴结转移者突变检出率明显高于无转移者（Ｐ＜０．０５）。结论喉癌ｐ５３基因突变与ＨＰＶ１６／１８感染有关，晚期喉癌颈淋巴结转移与ｐ５３基因突变有关。

Relationship between mutations of p53 in laryngeal carcinoma and HPV infection

Objective To investigate the relationship between mutations of p53 in laryngeal carcinoma and human papilloma virus(HPV) infection. Methods Using B solution system of Simpla spinning column,combining PCR-SSCP and PCR-DNA sequence, 36 patients with laryngeal carcinoma were detected for HPV typies, quantitative analysis of virus in infected cells and exon 4-8 mutations of p53 gene. Results The point and exchanging codon mutations were found in 13 cases,and 10 of those were infected with HPV16/18, gene mutations were positively correlated with virus number. In 24 cases with clinical - stage, the mutational rate with neck lymphatic matastasis was significant higher than that with no matastasis ( P <0.05). Conclusion Mutations of p53 gene is related with infection of HPV16/18 and neck lymphatic nodes matastasis in late stages of laryngeal squamous carcinoma.