以DNA测序及限制性酶切反应鉴定PCR产物特异性 |
| |
作者单位: | 1 Department of Immunology; 2 Department of Opthamlology,The ist Clinical Collgeg |
| |
摘 要: | 目的:对PCR产物进行特异性鉴定。方法:采用DNA测序及限制性酶切反应方法。结果:DNA测序可排除PCR产物的假阳性。另一种简便易行的方法是,设计包含某一已知偏位性酶切位点的特异引物进行PCR,通过酶切反应及电泳分析鉴定其特异性。结论:此方法对PCR产物特异性鉴定十分必要。
|
关 键 词: | 多聚酶链式反应(PCR) DNA序列分析 限制性酶切反应 |
Identify the specificity of PCR products by DNA sequencing and restriction endonuclease reactions |
| |
Authors: | Chang Yaping Qiao Yuan Zhao Shihong |
| |
Institution: | Chang Yaping1,Qiao Yuan1,Zhao Shihong2 |
| |
Abstract: | Objective:To identify the specificity of PCR products. Methods:DNA sequencing and restriction endonuclease reaction was used. Results: Our study indicated that pseudopositive results of PCR products were identified by DNA sequencing. Another convenient and applicable method was that PCR primers flanking a sequence with a asymmetrical restriction enzyme site was designed. Thus the PCR preducts could be identified by restriction endonuclease reaction. Conclusion: The study indicates that it is necessary to identify the specificity of PCR products.Kegwords: polymerase chain reaction (PCR);DNA Sequencing;restriction endonuclease reaction |
| |
Keywords: | polymerase chain reaction (PCR) DNA Sequencing restriction endonuclease reaction |
本文献已被 CNKI 等数据库收录! |