用荧光探针JC-1检测氧化应激对精子线粒体膜电位的影响

目的：利用荧光探针JC-1观察氧化应激对精子线粒体膜电位的变化。方法：用FeSO4/H2O2体系诱导精子氧化损伤模型，利用新型荧光探针JC 1标记精子线粒体,在FACS Calibur流式细胞仪上检测JC 1荧光强度的变化。结果：正常精子线粒体维持较高的膜电位,JC 1在线粒体内形成聚合物, 红色荧光占主导地位。FeSO4/H2O2体系导致线粒体膜电位下降, JC 1聚合物分解成单体,红色荧光强度减弱。结论：氧化应激导致精子线粒体膜电位下降; JC 1结合流式细胞仪是一种理想的检测线粒体膜电位的手段。

Detection of mitochondrial membrane potential changes in spermatzoa by fluorescent dyes JC-1

Objective: To explore the changes of mitochondrial membrane potential(MMP) in sperms in oxidative stess model with fluorescent JC-1.Methods: FeSO_4/H_2O_2 system was used to induce sperm model of oxidative injury,and JC-1 in combination with flow cyometry was used to detect the changes of sperm MMP in the exposure of reactive oxygen species(ROS).Results: Normal sperms had a high MMP,and JC-1 aggregates were formed in the inner membranes of mitochondria and emitted orange-red fluorescence.ROS caused the decrease of MMP,and JC-1 aggregates were dissociated to monomer which emitted green fluorenscence.Conclusions: Oxidative stress can cause the decrease of MMP;JC-1 in combination with flow cytometry is an ideal method to detect the change of mitochondrial membrane potential.