针刺“足三里”对大鼠胃血流影响的激光多谱勒血流成像的初步观察

目的:观察针刺对胃脏血流灌注的影响,探讨激光多谱勒血流成像技术在针灸内脏效应研究中的应用价值。方法:应用激光多谱勒血流灌注成像仪(LDPI)观察针刺大鼠“后三里”穴后胃脏血流灌注和微循环分布的改变。结果:激光多谱勒血流图对针刺前后的胃脏血流分布的改变显示清晰。自然状态下,胃脏激光多谱勒血流图呈现出从胃小弯向胃大弯放射状血流分布的扇形特征。不针刺对照组在观察的时间内胃部血流量有逐渐减少的趋势。针刺“后三里”穴后,胃脏血流灌注明显增加,除扇形分布的血管血流量较高外,其它部位的微循环血流量也明显增多,停针10 min时血流增加最多。结论:LDPI能以图像的形式显示针灸对胃脏的血管反应,该新技术方法在针灸内脏效应的研究方面将有一定的应用价值。

Primary Observation on the Effect of Acupuncture of "Zusanli" (ST 36) on Blood Flow of the Rat's Stomach by Using Laser Doppler Perfusion Imaging

Objective: To observe the effect of acupuncture of "Zusanli"(ST 36) on blood flow of the stomach in the rat in order to evaluate the applied value of laser Doppler perfusion imager(LDPI) in acupuncture study.Methods: Thirty-eight Wistar rats were randomized into control group(n=18) and acupuncture group(n=20).Under anesthesia by 2% pentobarbital((2.5 mg/kg)),the rat's stomach body was exposed and put on a test pad.Then the rat was put in a constant temperature-box by controlling the temperature to be within 30-(35 ℃) and the change of temperature in an experiment to be ±(1 ℃).After inserting the acupuncture needle into "Zusanli"(ST 36),it was manipulated for(1 min),then kept in place for(10 min).The probe of LDPI was set to aim at the stomach with the distance between the probe and the stomach being about(20 cm).The images were analyzed by using the instrument-installed LDPI 2.5 software.Results: Under natural state,blood flow shown by the images presented a flabellate distribution from the lesser curvature to the greater curvature of the stomach,and decreased gradually during the experiment in the control group.After acupuncture of "Zusanli"(ST 36),the results showed that there were distinct differences in the perfusion images flow(increase of blood flow,particularly at the 10~(th) min after acupuncture) on the middle part of the stomach and the region near the cardia in comparison with control group.Conclution: Acupuncture of "Zusanli"(ST 36) can increase the blood flow in the rat.The present finding demonstrates that LDPI can be used to provide an objective and specific assessment on the effects of acupuncture.