肝脾细胞输注在异种移植中的耐受诱导

目的 :观察肝脾细胞输注诱导异种胰岛移植的免疫耐受性。方法 :采用STZ制备BALB C小鼠糖尿病模型 ,经尾静脉注射供体猪肝细胞和 或脾细胞 3次后 ,腹腔内注射法进行猪胰腺细胞移植。测定血糖浓度变化 ,观察小鼠移植物有功能存活时间。选择常规方法测定小鼠移植后NK细胞活性变化 ,T细胞亚群和B细胞体外抗体形成实验。结果 :肝脾细胞联合胰岛移植组血糖在移植后 2 0d内均处于较低水平 ,并与同期其他各实验组的血糖有明显差异 (P <0 0 5 )。于 35～ 4 5d内出现移植物功能丧失。NK细胞杀伤活性移植后肝脾细胞联合胰岛移植组没有明显变化 (P >0 0 5 )。其他各实验组的NK细胞杀伤活性均明显升高 (P<0 0 5 ) ,T细胞亚群亦与NK细胞杀伤活性一样出现同样变化。脾脏B细胞体外溶血功能均增强 (P<0 0 5 )。结论 :少量多次供体肝细胞和脾细胞提前输注可以诱导异种胰岛细胞移植的免疫耐受性

Liver cells and/or spleen cells injection induce islets transplantation resistance

Objective:To observe the effect of liver cells and/or spleen cells injection induce islets transplantation resistance.Methods:New born male pigs and BALB/C mice were selected as donors and recipients respectively.The islets transplantation were performed in recipients just after tail vein injection with donor liver cells and/or spleen cells for 3 times.NK cells activity,antibodies forming function in vitro of B lymphocytes and T lymphocytes subsets measurement were used as immunological markers of transplantation resistance besides observation of the variation of blood glucose and xenograft living time(days).Results:The pre injection of donor liver cells,spleen cells or their mixture through mice tail vein was effective in preventing donor islets transplantation from rejection,which was demonstrated by the above immunological markers.And each kind of the transplantation could decrease the blood glucose of recipients and prolong the function possessed days of xenografts,especially for the more effective of mixture of donor liver cells and spleen cells as compared with the donor islets transplantation alone.Conclusion:Tail vein injection with donor liver cells and/or spleen cells could induce successfully islets transplantation resistance in mice.