| 人类白细胞抗原（HLA）基因分型在造血干细胞移植中的应用 |
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| 引用本文： | 刘川,邹叶青,李剑. 人类白细胞抗原（HLA）基因分型在造血干细胞移植中的应用[J]. 江西医学检验, 2008, 0(6): 587-588 |
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| 作者姓名： | 刘川 邹叶青 李剑 |
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| 作者单位： | 南昌大学第二附属医院分子医学实验室; |
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| 基金项目： | 江西省卫生厅科题(编号20043074) |
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| 摘 要： | 目的提高造血干细胞移植供、受者HLA配型的精确度，有效防止移植物抗宿主病Graft—vs host disease（GVHD）的发生。方法采用准确、简便的DNA提取方法和PCR—SSP基因分型方法。建立HLA—A，B，DR基因分型方法。结果对167例临床标本进行基因水平的研究发现。DNA提取方法可以满足此项研究对样本的要求。HLAPCR—SSP基因分型方法具有良好的稳定性、可靠性和特异性。结论HLA基因分型方法准确、特异、重复性好．可作为临床造血干细胞移植配型和正常人群无关供者筛选的常规方法。对（GVHD）的发生必将起到重要的预防作用。
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| 关 键 词： | 造血干细胞移植 人类白细胞抗原 PCR—SSP 移植物抗宿主病 |
Application of HLA genotyping method in hematopoietic stem cell transplantation |
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| LIU Chuan,ZOU Yeqing,LI Jian.Labortory of Molecular Medicine,The Second Affiliated Hospital of Nanchang University,Nanchang ,China. Application of HLA genotyping method in hematopoietic stem cell transplantation[J]. Jiangxi Journal of Medical Laboratory Sciences, 2008, 0(6): 587-588 |
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| Authors: | LIU Chuan ZOU Yeqing LI Jian.Labortory of Molecular Medicine The Second Affiliated Hospital of Nanchang University Nanchang China |
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| Affiliation: | LIU Chuan,ZOU Yeqing,LI Jian.Labortory of Molecular Medicine,The Second Affiliated Hospital of Nanchang University,Nanchang 330006,China |
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| Abstract: | Objecive To improve the accuracy of HLA typing method for hematopoietic stem cell transplantation of donor-recipientpairs,and to prevent graft vs host disease(GVHD).Methods An accurate,simple DNA extraction method and PCR-SSP genotyping method were employed for HLA-A,B,DR genotyping.Results The results of 167 cases by HLA DNA extraction with the PCR-SSP methods showed reliable,stable and specific.Conclusions HLA PCR-SSP can be used as routine test for hematopoietic stem cell transplantation and selecting un... |
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| Keywords: | Hematopoietic stem cell transplantation HLA PCR-SSP Graft-versus-host disease |
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