HPLC法测定酒石酸美托洛尔注射液的含量和有关物质

目的：建立HPLC法测定酒石酸美托洛尔注射液的含量和有关物质。方法：色谱柱：phenomenex luna C18柱（150mm×4．6mm，5μm），流动相：甲醇-庚烷磺酸钠缓冲溶液（水450ml，加庚烷磺酸钠960mg，加无水醋酸钠82mg，加冰醋酸0．57m1）（49：51），流速：1．0ml·min^-1，检测波长：275nm。结果：在选定的色谱条件下，主药与杂质分离良好。酒石酸关托洛尔在28．94～964．80μg·m^-1范围内线性关系良好（r=0．9999，n=6）。结论：该方法可用于酒石酸关托洛尔注射液的含量和有关物质的测定。

Determination of Metoprolol Tartrate Injection and Related Substances by HPLC

Objective： To estable a method for determination of metoprolol tartrate injection and related substances by HPLC. Method： The phenomenex luna C18 column （150 mm× 4.6 mm ,5 μm） was used, the mobile phase was consisted of methanolsodium heptanesulfonate buffer （ water 450 ml, adds sodium heptanesulfonate 960 mg, anhydrous sodium acetate 82 mg and acetic acid 0.57 ml）（49： 51） ,the flow rate was 1.0 ml · min^-1 and wavelength of detector was at 275 nm. Result： At the selected chromatographic conditions, the main drug was well separated from related materials and determined with a good linear relationship within the range of 28.94-964.80 μg · ml^-1（ r = 0. 999 9, n = 6 ）. Conclusion： The method can be used to determine the metoprolol tartrate and the related substances.