破伤风类毒素聚乳酸微球的体外释放研究 及附加剂对药物释放的影响

目的：研究破伤风类毒素聚乳酸微球(TT-MS)的体外释放规律，考察附加剂对药物释放的影响。方法：破伤风类毒素聚乳酸微球体外释放试验进行了84 d，测定释放量，考察: （1）聚乳酸平均相对分子质量;（2）微球载药量;（3）微球粒径;（4）附加剂对药物释放的影响。结果：破伤风类毒素聚乳酸微球体外释放有显著的长效作用，释放模型为零级释放，释放速度与前面提到的4种影响因素直接相关。结论：所研究的聚乳酸微球可望发展成为疫苗控释系统。

Study on the in vitro release of tetanus toxo id from polylactide microspheres and the influence of additives on the drug release

To study on in vitro release of tetanus toxoid contained in polylactide microspheres and the influence of additives on the drug release. Methods: Some parameters affecting the rate of release were assessed during the in vitro experiments lasted 84 days: (1)the molecular mass of the polymers, (2)the protein loading of the microspheres, (3)the particle sizes, (4)co encapsulation of additives. Results: The polylactide microspheres containing tetanus toxoid presented considerable sustained release effect. Release kinetics of the microspheres fitted in with zero order equations. The rate of release was shown to depend on the four parameters mentioned above. Conclusion: The polylactide microspheres investigated may be prospective for the development of controlled release vaccines.