Analysis of STR-PCR products with high-resolution denaturing PAGE |
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| Authors: | F Neuhuber M Radacher G Sorgo |
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| Institution: | (1) Institute of Legal Medicine, University of Salzburg, Ignaz-Harrer-Strasse 79, A-5020 Salzburg, Austria |
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| Abstract: | The Pharmacia Multiphor II horizontal electrophoresis chamber is a widespread tool for analysis of PCR products in forensic casework. Up to date, however, there is no protocol for successfully running high-resolution denaturing PAGE (poly-acrylamide gel electrophoresis) on a horizontal electrophoresis chamber. We modified the electrophoresis conditions to make this possible. |
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| Keywords: | Short tandem repeats DNA-polymorphisms Denaturing PAGE Horizontal electrophoresis |
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