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Characterization of a Swedish bovine isolate of Neospora caninum |
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| Authors: | S Stenlund C Björkman O J M Holmdahl H Kindahl A Uggla |
| Institution: | Department of Parasitology, National Veterinary Institute and Swedish University of Agricultural Sciences, Box 7073, S-75007 Uppsala, Sweden Fax:?+?46 - 18 30 91 62; e-mail: arvid.uggla@sva.se, SE Department of Cattle and Sheep Diseases, Swedish University of Agricultural Sciences, Box 7019, S-75007 Uppsala, Sweden, SE Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Box 7039, S-75007 Uppsala, Sweden, SE |
| Abstract: | The brain of a stillborn calf, seropositive to Neospora caninum and born to a seropositive cow, was homogenized and cultured on Vero cells, where growth of Neospora-like tachyzoites was detected after 8 weeks. The ultrastructural features of the new isolate (Nc-SweB1) corresponded to those of previously published Neospora isolates. In indirect immunofluorescence tests, antigens on Nc-SweB1 tachyzoites were recognized by antibodies raised to a canine N. caninum isolate (Nc-1) but not by antibodies to Toxoplasma gondii, Sarcocystis cruzi, S. tenella, Eimeria alabamensis, Babesia divergens, or B. motasi. Immunoblot analyses revealed no major antigenic difference between Nc-SweB1 and Nc-1, whereas several differences were seen between Nc-SweB1 and protozoa related to N. caninum. The sequences of 16S-like rRNA and the internal transcribed spacer 1 of Nc-SweB1 revealed complete homology with corresponding sequences of two canine N. caninum isolates. Thus, no dissimilarity between Nc-SweB1 and the canine isolates was found, confirming that Nc-SweB1 is N. caninum and suggesting that Neospora-like organisms isolated from cattle are indeed N. caninum. |
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