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| MIA PaCa-Ⅱ胰腺癌细胞系神经转移动物模型建立的实验研究 | |
| 引用本文： | 丁兆军,孟令新,陈希平,迟玉华,孟芹,李强,史玉荣,郝希山.MIA PaCa-Ⅱ胰腺癌细胞系神经转移动物模型建立的实验研究[J].实用肿瘤杂志,2009,24(2):118-120. |
| 作者姓名： | 丁兆军 孟令新 陈希平 迟玉华 孟芹 李强 史玉荣 郝希山 |
| 作者单位： | 1. 日照市人民医院肿瘤科,日照市肿瘤治疗中心,山东,日照,272826 2. 天津医科大学附属肿瘤医院腹部肿瘤科,天津,300060 3. 天津医科大学附属肿瘤医院国家肿瘤学"211工程"中心实验室,天津,300060 |
| 摘 要： | 目的建立MIA PaCa-Ⅱ人胰腺癌细胞系裸鼠胰腺原位移植瘤模型并观察神经侵袭情况。方法将MIA PaCa-Ⅱ细胞行裸鼠背部皮下接种,建立高转移特性胰腺癌裸鼠皮下移植瘤模型,然后将高转移性移植瘤组织接种于裸鼠胰腺被膜下,分别于4周、6周和8周处死裸鼠行移植瘤组织解剖和病理学检查,测量移植瘤大小和重量,并用HE和银染方法观察神经侵袭情况。结果MIA PaCa-Ⅱ细胞裸鼠皮下接种成瘤率为100.0%（10/10）,皮下移植瘤呈局限性生长,无脏器和神经转移。原位移植4周、6周和8周后,胰腺癌原位移植瘤成瘤率均为100.0%（10/10）,神经转移率分别为50.0%（5/10）、80.0%（8/10）和60.0%（6/10）,并可见多个脏器转移,以肝脏、肝门淋巴结、胃窦转移和腹膜播散多见。结论人胰腺癌细胞系MIA PaCa-Ⅱ裸鼠胰腺原位移植瘤模型为一较理想的＂拟人＂神经浸润转移模型,可用于胰腺癌体内嗜神经性机制的研究。 |
| 关 键 词： | 胰腺肿瘤/病理学 疾病模型 动物 肿瘤转移 肿瘤种植 肿瘤浸润 神经系统/病理学 |
Establishment of human pancreatic cancer model with perineural invasion in nude mice |
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| Institution: | DING Zhao-jun,MENG Ling-xin,CHEN Xi-ping,et al (Department of Oncology, Rizhao People＇s Hospital, Rizhao, 276826, China) |
| Abstract: | Objective To establish human pancreatic cancer model with perineural invasion in nude mice. Methods Human pancreatic cancer MIA PaCa-Ⅱ cells were inoculated subcutaneously in nude mice,then transplanted orthotopically into pancreas. Morphological and biological features, metastasis and nerve invasion of the transplanted tumor were studied after 4,6 and 8 weeks. Results The successful rate of orthotopic implantation of pancreatic cancer was 100.0% . The rate of nerve invasion was 50. 0%,80. 0% and 60. 0% after 4,6 and 8 weeks of transplantation. Conclusion Orthotopic human pancreatic cancer model with perineural invasion has been successfully established in nude mice. |
| Keywords: | pancreatic neoplasms/pathology disease models animal neoplasm metastasis neoplasm seeding neoplasm invasiveness nervous system/pathology |
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