A protective chimeric antibody to tick-borne encephalitis virus |
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| Authors: | Ivan K. Baykov Andrey L. Matveev Oleg V. Stronin Alexander B. Ryzhikov Leonid E. Matveev Marat F. Kasakin Vladimir A. Richter Nina V. Tikunova |
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| Affiliation: | 1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia;2. Federal State Unitary Company “Microgen Scientific Industrial Company for Immunobiological Medicines” of the Health Ministry of The Russian Federation, Branch in Tomsk, ul. Ivanovskogo 8, Tomsk 634040, Russia;3. Federal State Research Institution State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk Region 630559, Russia |
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| Abstract: | The efficiency of several mouse monoclonal antibodies (mAbs) specific to the tick-borne encephalitis virus (TBEV) glycoprotein E in post-exposure prophylaxis was assessed, and mAb14D5 was shown to be the most active of all those studied. It was proven that the hybridoma cell line 14D5 produced one immunoglobulin H chain and two L chains. They were used to construct chimeric antibodies ch14D5a and ch14D5b, the affinity constants of which were 2.6 × 1010 M−1 and 1.0 × 107 M−1, respectively, according to the SPR-based ProteOn biosensor assay. The neutralization index (IC50) of ch14D5a was 0.04 μg/ml in the focus reduction neutralization test. In in vivo experiments, ch14D5a at a dose of 10 μg/mouse resulted in a 100% survival of the mice infected with 240 LD50 of TBEV. This chimeric antibody is promising for further development of prevention and therapeutic drugs against TBEV. |
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| Keywords: | TBEV, tick-borne encephalitis virus ADE, antibody-dependent enhancement RU, response unit |
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