Vaccination of cattle with a recombinant bivalent toxoid against botulism serotypes C and D |
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| Authors: | Carlos E.P. Cunha,Gustavo M.S.G. Moreira,Felipe M. Salvarani,Monique S. Neves,Francisco C.F. Lobato,Odir A. Dellagostin,Fabricio R. Conceiç ã o |
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| Affiliation: | 1. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900 Pelotas, RS, Brazil;2. Escola de Veterinária, Universidade Federal de Minas Gerais, CP 567, CEP 30123-970 Belo Horizonte, MG, Brazil |
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| Abstract: | Cattle botulism is a fatal intoxication caused by botulinum neurotoxins (BoNTs) produced by Clostridium botulinum serotypes C and D resulting in economic losses. Vaccination is the most effective way to control botulism. However, the commercially available vaccines are difficult and hazardous to produce. Neutralizing antibodies against the C-terminal fragment of the BoNT heavy chain (HC) are known to protect against lethal doses of BoNTs. We report the vaccination of cattle with a previously tested recombinant chimera consisting of Escherichia coli heat-labile enterotoxin B subunit and the HC of BoNTs C and D. Vaccinated animals produced neutralizing antibodies against serotypes C and D averaging 5 ± 0 and 6.14 ± 1.06 IU/mL, respectively. For BoNT D, the titers were greater than those measured for the commercial vaccine, which induced titers of 5 ± 0 and 2.85 ± 1.35 against the respective serotypes, suggesting that this chimera is effective against cattle botulism. |
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| Keywords: | Recombinant vaccine Fused antigens Clostridium botulinum Botulinum neurotoxins LTB |
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