Immune responses of mice against recombinant bovine herpesvirus 5 glycoprotein D |
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Authors: | Luana Alves Dummer,Itauá Leston Araujo,Paula Fonseca Finger,Alceu Gonç alves dos Santos Jr.,Matheus Costa da Rosa,Fabricio Rochedo Conceiç ã o,Geferson Fischer,Sylvia van Drunen Littel-van den Hurk,Fá bio Pereira Leivas Leite |
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Affiliation: | 1. Laboratório de Bacteriologia, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS 96010-900, Brazil;2. Laboratório de Immunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS 96010-900, Brazil;3. Laboratório de Virologia e Imunologia Animal, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS 96010-900, Brazil;4. Microbiology & Immunology and VIDO-Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada |
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Abstract: | Glycoprotein D (gD) is essential for attachment and penetration of Bovine herpesvirus 5 (BoHV-5) into permissive cells, and is a major target of the host immune system, inducing strong humoral and cellular immune responses. The aim of this study was to evaluate in mice the immunogenicity of recombinant BoHV-5 gD (rgD5) expressed in Pichia pastoris. Vaccines formulated with rgD5 alone or adjuvanted with Montanide 50 ISA V2; Emulsigen or Emulsigen-DDA was administered intramuscularly or subcutaneously. Almost all formulations stimulated a humoral immune response after the first inoculation. The only exception was observed when the rgD5 was administered subcutaneously without adjuvant, in this case, the antibodies were observed after three doses. Higher titers of neutralizing antibodies were obtained with the three oil-based adjuvant formulations when compared to non-adjuvanted vaccine formulations. The rgD5 vaccine stimulated high mRNA expression levels of Th1 (INF-γ) and pro-inflammatory cytokines (IL-17, GM-CSF). The results demonstrated that the recombinant gD from BoHV-5 conserved important epitopes for viral neutralization from native BoHV-5 gD and was able to elicit mixed Th1/Th2 immune response in mice. |
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Keywords: | Pichia pastoris gD Vaccine Humoral response Cytokines |
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