血清COL4A3蛋白与非小细胞肺癌(NSCLC)患者临床病理特征及预后的相关性

 目的 探索血清COL4A3蛋白与非小细胞肺癌（non-small cell lung cancer，NSCLC）患者临床病理特征及预后的相关性。方法 ELISA试剂盒检测90例NSCLC患者和58名健康对照血清中COL4A3蛋白含量。ROC曲线分析血清COL4A3水平以预测NSCLC患者肿瘤转移的敏感性与特异性。Kaplan-Meier生存分析研究血清COL4A3蛋白表达与患者生存期的相关性。对NSCLC患者预后的相关性因素进行单因素和多因素分析。采用MTT实验和Transwell实验分别检测血清COL4A3蛋白对NSCLC细胞增殖和迁移能力的影响。结果 NSCLC患者血清COL4A3水平明显高于健康对照（P<0.001），且高表达COL4A3的患者总生存期（overall survival，OS）低于低表达患者（P=0.021）。在单因素分析中，NSCLC患者预后与肿瘤大小（P=0.015）、病理组织分级（P=0.042）、淋巴结转移（P=0.015）、临床分期（P<0.01）以及COL4A3表达（P<0.01）密切相关；在多因素分析中，NSCLC患者的预后与患者淋巴结转移（P=0.032）、临床分期（P<0.01）以及血清COL4A3水平（P=0.012）密切相关。COL4A3蛋白可显著增强体外NSCLC细胞增殖和迁移能力，并呈浓度依赖性。结论 血清COL4A3蛋白水平与NSCLC进展密切相关，可能是一种NSCLC患者预后不良的独立标志物。

Correlation between serum COL4A3 protein and clinicopathological features and prognosis in patients with non-small cell lung cancer (NSCLC)

Objective To explore the correlation between serum COL4A3 protein and clinicopathological features and prognosis in patients with non-small cell lung cancer (NSCLC).Methods The serum COL4A3 protein content of 90 NSCLC cases and 58 healthy controls was detected by ELISA kit,and the correlation between serum expression and clinicopathological features was statistically analyzed.The sensitivity and specificity of tumor metastasisin patients with NSCLC were predicted by the ROC curve analysis of serum COL4A3 levels.Kaplan-Meier survival analysis was used to study the correlation between serum COL4A3 protein expression and survival of patients.Univariate and multivariate analysis were used to reveal the relative factors with prognosis in patients with NSCLC.The effects of serum COL4A3 protein on proliferation and migration of NSCLC cells were detected by MTT assay and Transwell assay,respectively. Results The serum COL4A3 of NSCLC patients was significantly higher than that of healthy controls (P<0.001),and overall survival (OS) in patients with high expression of COL4A3 was significantly shorter than that of patients with low expression of COL4A3 (P=0.021).In univariate analysis,the prognosis of NSCLC patients was closely related to tumor size (P=0.015),pathological tissue grade (P=0.042),lymph node metastasis (P=0.015),clinical stage (P<0.01) and COL4A3 expression (P<0.01).While in multivariate analysis,lymph node metastasis (P=0.032),clinical stage (P<0.01) and serum COL4A3 level (P=0.012) was strongly associated with poor prognosis of NSCLC patients.In addition,the proliferation and migration capacity of NSCLC cells was significantly enhanced by COL4A3 protein in vitro in a dose-dependent manner.Conclusions Serum COL4A3 protein level is closely related to NSCLC progression,which may be an independent marker of poor prognosis in NSCLC patients.