| JAK2/STAT3途径在IL-6抑制LPS诱导的树突状细胞分化成熟中的作用 |
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| 引用本文： | 赵永亮,余佩武,雷晓,刘伟,饶芸.JAK2/STAT3途径在IL-6抑制LPS诱导的树突状细胞分化成熟中的作用[J].第三军医大学学报,2009,31(11). |
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| 作者姓名： | 赵永亮 余佩武 雷晓 刘伟 饶芸 |
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| 作者单位： | 第三军医大学西南医院全军普通外科中心,微创胃肠外科中心,重庆,400038 |
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| 摘 要： | 目的 观察JAK2/STAT3信号途径在IL-6抑制LPS诱导的小鼠髓源性树突状细胞(bone marrow-derived dendritic cells,BMDCs)分化成熟中的作用.方法 分离小鼠骨髓细胞,采用黏附法结合GM-CSF和IL-4刺激法在体外培养BMDCs;用相差倒置显微镜观察BMDCs的形态特点;用LPS诱导DCs成熟;采用流式细胞术观察IL-6处理与否,LPS诱导的BMDCs在表型上的变化;Western blot检测BMDCs细胞质p-JAK2、总STAT3和P-STAT3的表达水平.结果 形态观察和FCM分析的结果表明:体外成功培养了纯度较高的高表达CD11c的BMDCs,LPS能明显诱导BMDCs表面MHC-Ⅱ类分子和共刺激分子CD80、CD86、CD40的表达上调;而经过IL-6处理后,LPS诱导BMDCs表面MHC-Ⅱ类分子、CD80、CD86、CD40表达上调的能力被显著削弱(P＜0.01).Western blot结果显示,与单独用LPS处理的BMDCs相比,经IL-6处理后的BMDC中p-JAK2、P-STAT3表达随作用时间增加而明显上调,提示IL-6促进JAK2/STAT3信号途径活化.结论 JAK2/STAT3信号途径参与了IL-6抑制LPS诱导的BMDCs成熟过程.
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| 关 键 词： | 髓源性树突状细胞 白细胞介素-6 JAK2/STAT3信号途径 |
IL-6 suppresses LPS-induced DCs maturation via JAK2/STAT3 pathway |
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| ZHAO Yong-liang,YU Pei-wu,LEI Xiao,LIU Wei,RAO Yun.IL-6 suppresses LPS-induced DCs maturation via JAK2/STAT3 pathway[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(11). |
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| Authors: | ZHAO Yong-liang YU Pei-wu LEI Xiao LIU Wei RAO Yun |
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| Institution: | ZHAO Yong-liang,YU Pei-wu,LEI Xiao,LIU Wei,RAO Yun(Department of General Surgery,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To investigate the role of JAK2/STAT3 signaling pathway in IL-6-induced suppression during the maturation of mouse bone marrow-derived dendritic cells(BMDCs) after LPS treatment.Methods To obtain immature BMDCs,mouse bone marrow cells were isolated and cultured in complete culture medium containing GM-CSF and IL-4 after removing the nonadherent cells.The cell morphology was observed by using a phase contrast inverted microscope.LPS was added into the culture medium of BMDCs to induce the cell matu... |
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| Keywords: | LPS |
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