Genetic identification of cinnamon (Cinnamomum spp.) based on the trnL-trnF chloroplast DNA |
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Authors: | Kojoma Mareshige Kurihara Kogo Yamada Kazuya Sekita Setsuko Satake Motoyoshi Iida Osamu |
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Affiliation: | Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Shizuoka, Japan. kojoma@postman.riken.go. |
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Abstract: | Genetic identification among cinnamon species was studied by analyzing nucleotide sequences of chloroplast DNA from four species (Cinnamomum cassia, C. zeylanicum, C. burmannii and C. sieboldii). The two regions studied were the intergenic spacer region between the trnL 3'exon and trnF exon (trnL -trnF IGS) and the trnL intron region. We found nucleotide variation at one site in the trnL-trnF IGS, and at three sites in the trnL intron. With the sequence data from analysis of these regions, the four Cinnamomum species used in this study were correctly identified. Furthermore, single-strand conformation polymorphism (SSCP) analysis of PCR products from the trnL-trnF IGS and the trnL intron resulted in different SSCP band patterns among C. cassia, C. zeylanicum and C. burmannii. |
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