男性不育症患者中YRRM2基因缺陷的研究

YRRM基因是控制精子生成与成熟的一个重要因素，一旦该基因缺陷，将会造成无精或少精。本研究对340例无精与少精患者的外周血标本进行了PCR基因扩增筛查，结果发现7例为该基因缺陷，占2％。证明YRRM2的缺陷也是造成中国人群男性不育的一个原因，从而可作为指导医生采用适当治疗方法的一个可靠指标。在实验过程中，采用直接加热白细胞提取基因组DNA，并以此作为模板对YRRM2基因进行扩增，既节省时间和经费，又保持良好的扩增效果，使之可用于临床医院作为常规检查。

A study of the aberrations of YRRM genes among the infertile males by PCR method

The 2%-7% of the couples have not had a child at the end of their reproductive life. Among them, infertile males account for 40%. Of all men who seek help at infertility clinics, just over 10% are diagnosed to have oligo-or azoospermia of unknown etiology. The genes of YRRM family (Y chromosome located RNA recognition motif) are candidate genes that are important in spermatogenesis. The aberrations of these genes will result in oligo-or azoospermia. By using PCR amplification,we detected 7 cases of deletion among 340 infertile male (2%). It indicates that the aberration of the YRRM genes are also an etiology of infertility of the Chinese people. It can be used as a reliable way for doctor to choose the adequate therapy. In order to save time and money, we prepare the genomic DNA by heating the leukocyte directly and use it as template to amplify YRRM genes by PRC,which can be simly used as a routine examination in hospitals.