X线辐射对仔鼠大脑颞叶皮层结构及乙酰胆碱酯酶活性、Bax蛋白和表皮生长因子表达的影响

目的 探讨X线辐射对仔鼠大脑颞叶皮层结构及大脑乙酰胆碱酯酶(AChE)活性、颞叶Bax蛋白和表皮生长因子(EGF)表达的影响.方法 对160只仔鼠(出生6～7d)用不同吸收剂量(0Gy、1Gy、3Gy、5Gy、7Gy)X线进行全身辐射,分别在辐射后1d、5d、10d和20d,用比色法检测仔鼠大脑皮层AChE活性的变化,...

Effects of X-ray on the structure of cerebral temporal lobe cortex and activity of acetylcholinesterase and expresion of Bax and epidermal growth factor of filial mice

Objective To investigate effects of X-ray on the structure of cerebral temporal lobe cortex and acetylcholinesterase (AChE) activity of the brain and expression of Bax protein and epidermal growth factor (EGF) of cerebral temporal lobe cortex in filial mice． Methods One hundred and sixty filial mice were irradiated with different dosages (0Gy，1Gy，3Gy，5Gy，7Gy) of X-ray．The AChE activity was detected by colorimetry, expression of Bax protein and EGF of cerebral temporal lobe cortex were measured by immunohistochemistry，and the changes of cerebral temporal lobe cortex were observed by biomicroscopy． Results X-ray affected the development of cerebrum of filial mice． The number of Bax protein immunopositive cells in 1Gy group was lower and that of EGF was higher than that of the control group at the 5th-20th day after irradiation(EM>P/EM>>0.05)， whereas AChE activity was higher than that of the control group(EM>P/EM><0.05)． The number of EGF immunopositive cells and AChE activity in 3Gy group firstly decreased and then increased(EM>P/EM><0.05)，whereas that of Bax protein firstly increased then decreased (P<0.05)．The expression of Bax protein and EGF was strongly positive in 5Gy and 7Gy groups，and the number of their positive cells was higher and AChE activity was lower than that of the control group(EM>P/EM><0.01)．The thickness of temporal lobe cortex decreased and their structure was not clear．Compared with the control group，cell den