小鼠肾集合管发育中主细胞的形态学变化及水通道蛋白-2和-3的表达

目的 观察小鼠肾集合管发育过程中主细胞的形态学变化及水通道蛋白(AQP)-2、-3的表达,探讨AQP-2、-3与小鼠肾集合管主细胞发育的关系及作用.方法 应用透射电镜、免疫组织化学、免疫印迹技术并结合体视学方法,观察并检测胚龄16、18 d 胎鼠及生后1、3、7、14、21 d仔鼠肾集合管发育过程中主细胞的形态学变化及...

Morphology of principle cells and expression of aquaporin-2, -3 in collecting duct during mouse kidney development

Objective To observe the morphological changes of principle cells and the expressions of aquaporin（AQP）-2 and AQP-3 in collecting duct during mouse kidney development, so to investigate their relationships and effects. Methods Transmission electron microscopy, immunohistochemistry, Western blotting and stereological methods were used to detect the expressions of AQP-2, AQP-3 and principle cells in mouse kidney of embryonic day 16, 18 and postnatal day 1,3,7,14 and 21. Results The collecting duct principle cells occurred at embryonic day 18. Morphological structures of principle cells were well-developed during postnatal day 7 to 21. AQP-2 was apparently noticed on the apical plasma membrane and in the cytoplasm of principle cells. The expression of AQP-2 increased at postnatal day 1, and reached peak level at postnatal day 21. AQP-3 was expressed on the basolateral membrane of principle cells. The expressions of AQP-3 increased gradually and reached peak level at postnatal day 1. Conclusion AQP-2 and AQP-3 are very important for balance of water during development and maturation in mouse ki