靛红衍生物IF203通过线粒体途径诱导人肝癌HepG2细胞凋亡

目的 探讨靛红衍生物IF203诱导人肝癌HepG2细胞凋亡及线粒体凋亡途径.方法 体外培养HepG2细胞,应用倒置相差显微镜观察细胞形态变化;采用酸性磷酸酶法(APA)、流式细胞术(FCM)和免疫印迹法(Western blotting),检测IF203对HepG2细胞生长、细胞凋亡率、线粒体膜电位、Caspase-9...

IF203 induced apoptosis in human liver HepG2 cells through mitochondrial pathway

Objective To study the involvement of mitochondrial pathway in IF203 induced apoptosis in human liver HepG2 cells. Methods HepG2 cells were cultured in DMEM containing 10% fetal bovine serum at 37 ℃ in a humidified atmosphere with 5% COSUB>2/SUB>Inverted phase contrast microscope was used to detect the morphologic changes; Acid phosphatase assay(APA), flow cytometry（FCM）and Western blotting were used to detect the changes of cell viability, apoptosis ratio, mitochondrial transmembrane potential, the expressions of apoptosis-related protein Caspase-9, Caspase-3 and cytochrome C. Results Cell growth was inhibited after HepG2 cells were co-cultured with different concentration of IF203 for 24 hours and 48 hours. HepG2 cells showed round and shrank after they were co-cultured with IF203 of 10 mg/L. Apoptosis ratios caused by IF203 were increased. Mean fluorescence intensity (MFI) of mitochondrial transmembrane potentials in HepG2 cells decreased. The expressions of apoptosis-related protein Caspase-9 and Caspase-3 decreased, but the cytochrome C expression increased. Conclusion IF203 could significantly restrain cell growth of liver cancer cell line HepG2- IF203 can activate mitochondrial signaling pathway which would induce apoptosis, b